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Short peptide suppressing growth of cancer cell and uses thereof

A cancer cell, short peptide technology, applied in the field of biotechnology and medicine, can solve the problems of pain, death, damage to normal cells of patients, etc., and achieve the effect of solving serious injuries

Active Publication Date: 2008-07-23
FUJIAN LANHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, As 2 o 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity. Whether one chemotherapy drug is used alone or several chemotherapy drugs are used in clinical treatment, It has obvious curative effect on cancer, while killing cancer cells, it will also damage the patient's normal cells
The most common side effect of chemotherapy drugs is the extreme suppression of the patient's bone marrow, causing great pain to the patient, and even death
So far, in addition to early detection of cancer and surgical resection of tumor masses, there is still no specific drug to treat spread tumors and leukemia [Cancer Biology, written by R.J.B Jin, translated by Liu Yixun, Science Press, 2002 ], most of the existing clinically used drugs for cancer treatment are chemical compounds, while the application of short peptides in cancer has not been reported in the literature

Method used

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  • Short peptide suppressing growth of cancer cell and uses thereof
  • Short peptide suppressing growth of cancer cell and uses thereof
  • Short peptide suppressing growth of cancer cell and uses thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0018] The preparation method of the short peptide adopts the chemical synthesis method, that is, the solid-phase peptide synthesis method which is well known in the art and has been very mature, and can adopt either the Boc method or the Fmoc method. The specific method is to couple the protected amino acids to an inert solid support one by one, and then use a strong acid to cleave the peptide chain from the support and remove the side chain protection at the same time.

[0019] The amino acid main sequence of the short peptide is the main sequence with fixed amino acid positions, and the corresponding amino acids are arranged in the spaced positions. At the position of J in , no matter which one of them is selected, the short peptide still has its original biological activity, that is, inhibiting the growth of malignant tumor cells. Similarly, the amino acids at the positions represented by B and O also have such properties. .

[0020] Nucleotides are prepared by synthetic ...

Embodiment 1

[0038] Test for inhibiting liver cancer cells (synthesis of short peptides involved in the present invention: Lys-Leu-Pro-Ser-Ser-Ala-Lys or Lys-Leu-Pro-Ser-Ala-Ala-Lys)

[0039] Human hepatoma cell line Bel-7402 cells were cultured in RPMI 1640 medium containing 10% newborn bovine serum at 5 × 10 5 The cell density per well was planted into a 96-well cell plate. After 24 hours, the short peptides involved in the present invention were added to the cell culture plate according to different concentrations. An equal volume of PBS was used as a control group. After 24 hours, a cell counting plate was used. Count, compare the administration group and the control group, and obtain the inhibition rate of liver cancer cells. like figure 1 As shown in the photos taken under the microscope 24 hours after the short peptide and human liver cancer cells involved in the present invention act together, the cells in the administration group Test can obviously observe a significant inhibitor...

Embodiment 2

[0041] Inhibition of acute myeloid leukemia (M 2 ) (synthesis of the short peptide derivatives involved in the present invention, modified with a phosphate group at serine: Thr-Leu-Pro-Serp-Ala-Ala-Lys)

[0042] The human acute myeloid leukemia cell line HL60 cells were cultured in RPMI 1640 medium containing 10% newborn bovine serum at 10 6 After the cell density per well was planted into a 24-well cell plate, the short peptides involved in the present invention were immediately added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as the control group. The existing clinical drug As 2 O 3 As a positive control, after 24 hours, after flow cytometry (FACS), the experimental results are as follows figure 2 shown, where the upper right 59.32% represents the number of dead cells and 38.91% represents the number of early apoptotic cells, the total effective rate of cells being inhibited exceeds 98%.

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Abstract

The invention provides a short peptide capable of inhibiting the growth of cancer cell, which is characterized in that the identity and similarity of the amino acid main sequence of the short peptide fragments, the analogues, the derivatives and the variants of the short peptide to the amino acid main sequence of the short peptide are greater than or equal to 70% and 90% respectively; the short peptides, the nucleotides, the short peptide fragments, the short peptide analogues, the short peptide derivatives and the short peptide variants can be used in preparing drugs for curing leukaemia caused by various lesion cell proliferation of non-solid tumor human myeliod line, curing various cancers caused by human solid tumor cancer cell proliferation, inhibiting hypertension, and diagnosing and detecting the occurrence and the malignancy of cancers; the short peptides and the nucleotides also can be used in preparing drugs which are used as protease inhibitor, accelerator, affinity reagent, or detection reagent. The short peptide capable of inhibiting the growth of cancer cell has an advantage of overcoming the defect of serious harmful effect on the normal cell of human body when the existing cancer drugs are used for inhibiting or killing the cancer cell.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a short peptide for inhibiting the growth of cancer cells and its application. Background technique [0002] In the process of cancer occurrence and treatment, chemotherapy has always been the main part. Existing clinical chemotherapy drugs, such as cyclophosphamide, cisplatin, cytarabine, doxorubicin, and vincristine, are mainly used to disrupt the cell cycle, including RNA, DNA and protein. drug-like targets. In recent years, As 2 O 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapeutic drugs have their own toxicity, no matter whether a chemotherapeutic drug is used alone or a combination of several chemotherapeutic drugs is used in clinical treatment, It has obvious curative effect on cancer, kills and kills cancer cells, but also damages normal cells of patients. The most common side effect of c...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N15/11C07H21/04A61K38/08A61K31/7088A61P35/00A61P35/02A61P9/12G01N33/68G01N33/574C12Q1/68
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH
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