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Short peptide suppressing growth of cancer cell and uses thereof

A technology of cancer cells and short peptides, applied in the field of biotechnology and medicine, can solve problems such as patient pain, death, chemotherapy drug bone marrow suppression, etc., and achieve the effect of solving serious injuries

Active Publication Date: 2010-10-20
FUJIAN LANHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, As 2 o 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity. Whether one chemotherapy drug is used alone or several chemotherapy drugs are used in clinical treatment, It has obvious curative effect on cancer, while killing cancer cells, it will also damage the patient's normal cells
The most common side effect of chemotherapy drugs is the extreme suppression of the patient's bone marrow, causing great pain to the patient, and even death
So far, in addition to early detection of cancer and surgical resection of tumor masses, there is still no specific drug to treat spread tumors and leukemia [Cancer Biology, written by R.J.B Jin, translated by Liu Yixun, Science Press, 2002 ], most of the existing clinically used drugs for cancer treatment are chemical compounds, while the application of short peptides in cancer has not been reported in the literature

Method used

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  • Short peptide suppressing growth of cancer cell and uses thereof
  • Short peptide suppressing growth of cancer cell and uses thereof
  • Short peptide suppressing growth of cancer cell and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.

[0019] The main amino acid sequence of the short peptide is a main sequence with fixed amino acid positions, and the corresponding amino acids are selected and arranged at the interval positions. For example, there are 3 kinds of Lys, Thr or Ile in the amino acid position of the J position, and these 3 kinds of amino acids can be placed in a single place. At the J position in , no matter which one is selected, the short peptide still has its original biological activity, that is, inhibits the growth of malignant tumor cells, a...

Embodiment 1

[0037] Test of inhibiting liver cancer cells (synthesis of the short peptide involved in the present invention: Lys-Leu-Pro-Ser-Ser-Ala-Lys or Lys-Leu-Pro-Ser-Ala-Ala-Lys)

[0038] The culture of the human liver cancer cell line Bel-7402 cells was cultivated with RPMI 1640 medium containing 10% newborn bovine serum, and was cultured at 5×10 5 The cell density per well was planted into a 96-well cell plate. After 24 hours, the short peptides involved in the present invention were added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as the control group. After 24 hours, the cell counting plate was used to Count, compare the administration group with the control group, and obtain the inhibition rate of liver cancer cells. As shown in Figure 1, the short peptide involved in the present invention and the photo taken under the microscope after the joint action of human liver cancer cells for 24 hours, the cells in the administrat...

Embodiment 2

[0040] Inhibition of acute myeloid leukemia (M 2 ) (synthesis of the short peptide derivatives involved in the present invention, modified by adding phosphoric acid group at serine: Thr-Leu-Pro-Serp-Ala-Ala-Lys)

[0041] Human acute myeloid leukemia cell line HL60 cells were cultured with RPMI 1640 medium containing 10% newborn bovine serum, and cultured in 10 6 After the cell density per well was planted into a 24-well cell plate, the short peptides involved in the present invention were immediately added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as a control group. The existing clinical drug As 2 o 3 As a positive control, after 24 hours, through flow cytometry detection (FACS), the experimental results are as follows figure 2 As shown, the right upper limit 59.32% represents the number of dead cells, 38.91% represents the number of early apoptotic cells, and the total effective rate of cell inhibition exceeds 98%...

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Abstract

The invention provides a short peptide capable of inhibiting the growth of cancer cell, which is characterized in that the identity and similarity of the amino acid main sequence of the short peptide fragments, the analogues, the derivatives and the variants of the short peptide to the amino acid main sequence of the short peptide are greater than or equal to 70% and 90% respectively; the short peptides, the nucleotides, the short peptide fragments, the short peptide analogues, the short peptide derivatives and the short peptide variants can be used in preparing drugs for curing leukaemia caused by various lesion cell proliferation of non-solid tumor human myeliod line, curing various cancers caused by human solid tumor cancer cell proliferation, inhibiting hypertension, and diagnosing and detecting the occurrence and the malignancy of cancers; the short peptides and the nucleotides also can be used in preparing drugs which are used as protease inhibitor, accelerator, affinity reagent, or detection reagent. The short peptide capable of inhibiting the growth of cancer cell has an advantage of overcoming the defect of serious harmful effect on the normal cell of human body when the existing cancer drugs are used for inhibiting or killing the cancer cell.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a short peptide for inhibiting the growth of cancer cells and its application. Background technique [0002] Chemotherapy has always been the main part in the process of cancer occurrence and treatment. The existing clinical chemotherapeutic drugs, such as cyclophosphamide, cisplatin, cytarabine, doxorubicin and vincristine, etc., are mainly used to destroy the cell cycle, and RNA, DNA and protein are all of them. target of drugs. In recent years, As 2 o 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity. Whether one chemotherapy drug is used alone or several chemotherapy drugs are used in clinical treatment, It has obvious curative effect on cancer, while killing and killing cancer cells, it will also damage the normal cells of the patient. The most common side effect ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12N15/11C07H21/04A61K38/08A61K31/7088A61P35/00A61P35/02A61P9/12G01N33/68G01N33/574C12Q1/68
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH
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