FQ15 enterococcus faecalis and method for producing somatotrophic feed additive with the bacteria
A feed additive and Enterococcus faecalis technology, applied in animal feed, animal feed, bacteria, etc., can solve the problems of poor weight gain effect of micro-ecological feed additives, achieve intestinal flora recovery balance, simple production process, and reduce use Effect
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Embodiment 1
[0035] A FQ15 Enterococcus faecalis CGMCC 2196 belongs to the genus Enterococcus and Enterococcus faecalis species, the strain code is FQ15, and the Latin name is Enterococcus faecalis.
[0036] Isolation and cultivation method of FQ15 Enterococcus faecalis strain:
[0037] After the healthy chickens were killed and dissected, 1 g each of the small intestinal mucosa and intestinal contents or fresh feces were taken by aseptic method, and added to a small bottle filled with 9 mL of sterilized normal saline, mixed evenly and then diluted to a ratio of 10 -4 、10 -5 、10 -6 100 μL of the diluted suspension was added dropwise to three MRS solid medium, spread evenly with a glass rod, and then cultured anaerobically and aerobically at 37°C for 48 h to observe the colony morphology and whether there were calcium-dissolving rings. Select lactic acid bacteria colonies with calcium-dissolving rings and inoculate them on MRS agar slant for pure culture, and store them in a refrigerator ...
Embodiment 2
[0046] The strains used are the same as in Example 1.
[0047] The difference from Example 1 is that the improved MRS medium in the c step is added with 5-30 g of soybean peptone, 1-10 g of glucose, 1-10 g of yeast powder, and 1-10 g of sodium acetate in every liter of MRS medium. Diamine citrate 0.1~8g, Tween80 0.1~5g, dipotassium hydrogen phosphate 0.1~5g, magnesium sulfate 0.05~1g, manganese sulfate 0.001~0.2g, calcium carbonate 1~30g, purified water 1 liter, adjust the pH value to 5.5~ 8.0.
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