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Technique for producing water-solubility function chitosan

A functional shell and water-soluble technology, which is applied in the production of water-soluble functional chitosan technology, can solve the problems of low water-soluble chitosan content, wide average molecular weight distribution, and limited application of physical methods, and achieve mild enzymolysis control parameters , less side reactions, longer industrial process

Active Publication Date: 2010-06-16
YANGZHOU RIXING BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of water-soluble chitosan by physical degradation is limited by the degradation mechanism. During the degradation process, the polymer chains of chitosan are randomly broken, and the average molecular weight distribution of the product is too wide. It is difficult to obtain products with a molecular weight below 40,000. The required degree of polymerization The water-soluble chitosan content of 6-8 is low, resulting in a large waste of raw materials, which greatly limits the application of physical methods

Method used

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  • Technique for producing water-solubility function chitosan
  • Technique for producing water-solubility function chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 50g chitosan, then add 50mL H 2 o 2 (18% concentration) and mix thoroughly, adjust the microwave power to 450W, the microwave heating time is 3min, take out and cool naturally. Add water to prepare a 15% chitosan solution, adjust the pH to 5-6 with acetic acid, add 3% chitosan-quality cellulase, react in a water bath at 55°C for 3 hours, then rapidly heat to 80°C for 10 minutes to inactivate the enzyme. The enzymatic solution is filtered and centrifuged to remove the precipitate, and the supernatant is subjected to membrane ultrafiltration with a molecular weight cut-off of 5000D, the operating pressure is 1.0Mpa, and the operating temperature is 50±1°C. 1°C, remove salt and small molecular monosaccharides, concentrate and control the filtrate concentration to 25±2%, and spray dry to make a product, which can be directly dissolved in water to form a transparent solution.

Embodiment 2

[0035] Weigh 50g chitosan, then add 60mL H 2 o 2(18% concentration) and mix thoroughly, adjust the microwave power to 450W, the microwave heating time is 3min, take out and cool naturally. Add water to prepare a 20% chitosan solution, adjust the pH to 5-6 with hydrochloric acid, add 2% chitosan-quality lipase, react in a water bath at 45°C for 5 hours, then rapidly heat to 80°C to inactivate the enzyme for 10 minutes. The enzymatic solution is filtered and centrifuged to remove the precipitate, and the supernatant is subjected to membrane ultrafiltration with a molecular weight cut-off of 10000D, the operating pressure is 1.0Mpa, and the operating temperature is 50±1°C. 1°C, remove salt and small molecular monosaccharides, concentrate and control the filtrate concentration to 25±2%, and spray dry to make a product, which can be directly dissolved in water to form a transparent solution.

Embodiment 3

[0037] Weigh 50g chitosan, then add 50mL H 2 o 2 (18% concentration) and mix thoroughly, adjust the microwave power to 450W, the microwave heating time is 3min, take out and cool naturally. Add water to prepare a 15% chitosan solution, adjust the pH to 5-6 with acetic acid, add papain with 3% chitosan quality, react in a water bath at 60°C for 4 hours, then rapidly heat to 90°C to inactivate the enzyme for 10 minutes. The enzymatic solution is filtered and centrifuged to remove the precipitate, and the supernatant is subjected to membrane ultrafiltration with a molecular weight cut-off of 3000D, the operating pressure is 0.8Mpa, and the operating temperature is 55±1°C. 1°C, remove salt and small molecular monosaccharides, concentrate and control the filtrate concentration to 25±2%, and spray dry to make a product, which can be directly dissolved in water to form a transparent solution.

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Abstract

The invention discloses a technique for producing water-soluble functional chitosan. The technique comprises the steps that: chitosan is added into H2O2, and the mixture is mixed evenly, microwave-heated for a proper time, and is cooled; the chitosan treated by microwave is added into the solutions of acetic acid or hydurochloric, and is stirred until the chitosan is fully dissolved; chitosan solution is prepared after pH is adjusted to 5 to 6; non-specific chitosanase is added into the chitosan solution, and zymohydrolysis, enzyme-annihilation, enzymolysis liquid filtering and centrifugalization are carried out to remove precipitate, and then a clear solution is obtained; hyperfiltration is carried out on the clear solution, and nanofiltration is carried out to the hyperfiltrated solutionto remove salinity and micromolecule monosaccharide; filtrate is sprayed and dried, and then the product is obtained. The technique organically integrates microwave degradation, oxidative degradationand zymohydrolysis and adopts non-specific enzyme to solve ab enzyme preparation supply problem; the controlling parameters of zymohydrolysis is comparatively moderate; the technique is applicable tothe scale production of water soluble functional chitosan.

Description

technical field [0001] The invention belongs to the field of chitosan production and relates to a process for producing water-soluble functional chitosan by microwave and enzymatic methods. Background technique [0002] Chitosan has a wide range of biological effects, especially the effects of improving the body's immune function, anti-tumor, and anti-infection, but because they are insoluble in water, their development and utilization have been greatly restricted. In contrast, water-soluble Chitosan has good water solubility, and at the same time has the functions of regulating the body's immune function and anti-tumor. [0003] The antibacterial activity test also showed that the antibacterial effect gradually increased with the decrease of the relative molecular mass of water-soluble chitosan, especially when the relative molecular mass was about 1500, the antibacterial effect was better. Comparative experiments also proved that the existence of free amino groups is the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/00C08L5/08C12S3/00
Inventor 张和张卫明戚善龙杨生桂张超史劲松孙达峰
Owner YANGZHOU RIXING BIO TECH
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