Biological pesticide using psoralidin to prevent and control plant fungal diseases
A technology for plant fungal diseases and psoralen, which is applied in the directions of plant growth regulators, chemicals for biological control, biocides, etc. Good inhibitory activity, high efficacy and broad bactericidal spectrum
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example 1
[0015] Example 1: Using acetone or dimethyl sulfoxide to dissolve Psoralidin (Psoralidin) to prepare a 10g / L crude extract mother liquor. Add an appropriate amount of crude extract mother liquor to PDA medium at 50℃, mix well, and prepare the final concentration of crude extract to be 0.12mg / L, 0.6mg / L, 3mg / L, 15mg / L, 75mg / L. The drug-containing medium is added to the PDA medium with an equal volume of acetone or dimethyl sulfoxide as a control, and then poured into a petri dish with a diameter of 90 mm to make a flat plate for use. A 6mm diameter hole punch was used to cut the edge of each test pathogen colony that had been cultured on a PDA plate at 28°C for 7-12 days, and transplanted it to the center of the above-mentioned crude extract plate and control plate containing different concentrations. This treatment was repeated 3 times, cultured at 28°C for 7-12 days, and the colony diameter was measured. The diameter of each colony is measured in the direction of the intersecting...
example 2
[0018] Example 2: The test method is the same as that of Example 1. The inhibitory effects of the six original medicines of psoralen, thiram, Yintai, thiophanate methyl, and carbendazim on the growth of the mycelium of apple rot fungi are as follows:
[0019] Inhibition of 5 kinds of medicaments on mycelium growth of apple rot disease fungus
[0020] Inhibition
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