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Liquid culture medium for culturing mematophagous fungi and method for preparing chlamydospore of the same

A technology of nematophilic fungi and liquid culture medium, applied in the direction of fungi, etc., can solve the problem of high production cost, achieve low cost, reduce production cost, and improve the level of production and industrialization

Inactive Publication Date: 2008-07-02
王治才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this liquid medium is expensive to prepare and can only be used for laboratory cultivation of this strain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: 1. The main raw materials of this liquid culture medium are potatoes and trace salts. Potatoes are peeled, broken, added water to ripen and filter to remove slag, and the content of potato juice is 0.5-20%; various inorganic salts, trace salts Contents are: KH 2 PO 4 0.5.0-2.0g / L; MgSO 4 .7H 2 O0.3-1.5g / L; KCI 0.3-1.50g / L; FeSO 4 .7H 2 O 0.005--0.02g / L; ZnSO 4 .7H 2 O 0.005--0.02g / L; CuSO 4 .5H 2 O 0.003--0.008g / L. The pH of the medium is 5.5-7.5.

[0016] 2. Use the liquid medium to prepare the seeds of the nematophilic fungus Duddingtonia flagrans: take the culture of the nematophilic fungus Duddingtonia flagrans stored on the agar culture plate, inoculate it into a 100-250ml Erlenmeyer flask equipped with the above medium and autoclaved Put it in a constant temperature shaking incubator at 25°C-30°C, shake it for 30-60 minutes every day for the first 5 days, and cultivate it statically for the rest of the time, or directly shake it for 10-14 ...

Embodiment 2

[0022] Embodiment 2: In embodiment 1:

[0023] 1. Weigh 200g of washed and peeled fresh potatoes, cut into thin slices, and stir into a paste. Add an appropriate amount of water and boil on low heat for 30 minutes. Filter through double gauze. The filtrate was supplemented with water to 1000ml and left to stand for 12 hours.

[0024] 2. Preparation containing KH 2 PO 4 1.0g, MgSO 4 .7H 2 O 0.5g, KCI 0.5g, FeSO 4 .7H 2 O0.01g, ZnSO 4 .7H 2 O 0.01g, CuSO 4 .5H 2 O 0.005g trace salt solution 1000ml.

[0025] 3. Take 100ml of the supernatant of potato juice after standing for 12 hours, 100ml of the above-mentioned trace salt solution, make up water to 1000ml, and adjust the pH to 6.5-7.5.

[0026] 4. Divide the above medium into 100-1000ml Erlenmeyer flasks or 5-15L fermenters. The filling capacity is 1 / 3-1 / 5 of the volume of the container, preferably 1 / 4; the mouth of the triangular bottle is plugged with a breathable silicone plug, and then the mouth of the bottle i...

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Abstract

A liquid culture medium for culturing Duddingtonia flagrans and a preparation method of chlamydospore thereof belongs to the field of prevention and treatment technology of gastrointestinal parasitic disease of livestock. The liquid culture medium mainly contains potato juice and trace amount of salt, and is filled in a 100 to 1,000 mL triangular flask or a 5 to 15 L fermentation tank by a volume of 1 / 3 to 1 / 5 of the container volume. The liquid culture medium can be used for preparing seeds of Duddingtonia flagrans and enlarging fermentation production after high-pressure sterilization. The technology has abundant and cost-effective raw materials, which facilitates the reduction of production cost, the alleviation of labor intensity, the improvement of a strain preparation as well as the improvement of scale production and industrialization level of the preparation. The technology can be used for fermentatively culturing Duddingtonia flagrans, which can provide seeds for batch production of chlamydospore of Duddingtonia flagrans and can be directly used for the production of chlamydospore.

Description

1. Technical field [0001] The present invention relates to a nematophilic fungus strain for the biological control of animal gastrointestinal nematode disease - a liquid culture medium and culture technology for the cultivation of Duddingtonia flagrans, belonging to the technical field of livestock gastrointestinal parasitic disease prevention and control, in particular to nematophilic fungus A cultured liquid culture medium and a method for preparing spores thereof. 2. Background technology [0002] At present, the prevention and treatment of gastrointestinal parasitic diseases in cattle and sheep mainly involves regular deworming of animals with various chemical drugs. Long-term repeated medication, on the one hand, leads to the accumulation of drugs in the livestock body, which reduces the quality of animal products; Parasite resistance is one of the biggest threats to the livestock industry globally. Therefore, research on biological control of parasitic diseases in li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 王治才王琳
Owner 王治才
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