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Use of a griffonia extract, in particular griffonia simplicifolia, in a cosmetic or dermatological composition for mitigating pigmentation of skin and skin appendages

A technology for pigmentation, extracts, applied in the field of Ghana tree, which can solve the problems of insufficient efficacy, difficulty in use, melanocyte cytotoxicity, etc.

Inactive Publication Date: 2008-06-11
AM PHYTO CONSEIL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the aforementioned substances are sometimes cytotoxic for melanocytes, and / or are not effective enough, and / or are difficult to use: for example, compositions based on hydroquinone are currently banned from free trade

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Obtaining the extract of the seeds of the simple leaf class Gardner tree

[0033] An extract of the seeds of Gardneria unifoliate is prepared in a known manner with the aid of methanol and water.

[0034] Starting from 500 g of seeds, 110 g of dry extract were obtained with the aid of 4 liters of a 50% water-methanol mixture.

[0035] Then, 2.5 g of this dry extract were dissolved in 250 ml of cold water and extracted twice with 100 ml of n-butanol:

[0036] - evaporation of the organic phase to obtain the butanol fraction;

[0037] - The aqueous phase is dried in a rotary evaporator and the dry residue is redissolved in 12 ml of boiling water to obtain 270 mg of the recrystallized aqueous fraction after filtration and cooling.

Embodiment 2

[0038] Example 2: Chromatographic comparison using 5-HTP

[0039] The dry extract obtained in Example 1, the butanol fraction and the recrystallized aqueous fraction were subjected to thin-layer chromatography in the presence of a 5-hydroxy-tryptophan (5-HTP) control using a volume ratio of 8 : 8:4:1 mixture of acetone / chloroform / acetic acid / water as eluent and ninhydrin in butanol as developer.

[0040] This analysis showed that 5-HTP was present in the dry extract, the butanol fraction, and the recrystallized aqueous fraction.

Embodiment 3

[0041] Example 3: Effect of extract on melanogenesis activity

[0042] The effect of the three extract products of Grizonia unifoliata obtained in Example 1 on the biosynthesis of melanin was investigated.

[0043] (a) Effect of dry extract

[0044] Normal human melanocytes were cultured for a period of 10 days in the absence or presence of this dry extract in non-cytotoxic amounts. The amount of melanin was estimated by 0.5N sodium hydroxide and optical density measurements.

[0045] This dry extract showed significant pigmentation inhibitory activity with p less than 0.01 at a concentration of 0.004 mg / ml.

[0046] (b) Effect of the butanol moiety

[0047] Proceed with the same procedure as for the previous dry extract.

[0048] The butanol fraction at a concentration of 0.04 mg / ml resulted in a significant reduction in the amount of melanin produced by melanocytes; only 86% of the amount obtained in the control culture was found (p less than 0.01).

[0049] (c) Effec...

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Abstract

The invention concerns the use of a Griffonia extract, in particular Griffonia simplicifolia, in a cosmetic or dermatological composition for mitigating pigmentation of skin and skin appendages. Said cosmetic or dermatological composition may also contain one or other of the following products: at least one active depigmenting product, at least one substance for filtering or blocking ultraviolet B and / or A rays, at least one anti-inflammatory product, at least one desquamation promoting agent. It contains 0.05 to 20 wt. % of a Griffonia simplicifolia extract.

Description

technical field [0001] The present invention relates to the use of extracts of Griffonia, especially Griffonia simplicifolia, in cosmetic or dermatological compositions for reducing pigmentation of the skin and epidermal tissues. Background technique [0002] The monophyll Ghana tree is a tree that grows mainly in West Africa, especially in Ghana, Côte d'Ivoire and Togo. Its stems are used topically for intercostal pain, while its leaves can be used for liver disease and antiemetic (Plantes Médicinales d'Afrique, J-L, Pousset, Edisud, 2004). The seeds of the unifoliate Griffonia tree are currently the only source used for the industrial production of 5-hydroxy-tryptophan, or 5-HTP. As described in the article in Nutranews, July 2004 and in Phytochem.Anal.13, 333-337 (2002), this molecule is used in medicine as an antidepressant and antiepileptic, and in the field of food additives is used as a precursor to serotonin, which is used to treat headaches, appetite or sleep diso...

Claims

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Application Information

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IPC IPC(8): A61Q19/02A61K8/97
CPCA61K8/97A61Q19/02A61K8/9789A61K8/9794
Inventor A·梅贝克
Owner AM PHYTO CONSEIL
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