Method for preparing pathogenicity hydrosphere unit cell bacterium killed vaccine

Abstract

The invention discloses a method for preparing pathogenicity aeromonas hydrophila bacteria inactivating vaccine, which comprises the following steps: firstly, inoculating the pathogenicity aeromonas hydrophila bacteria separated from the brown paralichthys olivaceus with abdominal dropsy through PCR detecting to the common LB culture medium to be cultured so as to reach a certain density; secondly, inactivating the bacteria by using formaldehyde solution; thirdly, collecting the thalli by using centrifugal culture and then diluting the thalli by using physiological saline so as to get the pathogenicity aeromonas hydrophila bacteria inactivating vaccine. The preparing method is rapid, simple and convenient in operation, and easy in batch preparing; the gotten vaccine is a pure biological agent which does not has any toxic and side effect and does not cause pollution to the meat quality of the paralichthys olivaceus and the surrounding water. The immunity protecting rate reaches 80 percent by using the vaccine prepared by the method to inoculate brown paralichthys olivaceus so as to radically curb the spreading of the pathogeny. The invention provides new approach for preventing and curing the epidemic abdominal dropsy of brown paralichthys olivaceus.

Description

technical field [0001] The invention relates to a method for preparing a biological preparation, in particular to a method for preparing an inactivated vaccine of pathogenic Aeromonas hydrophila, and belongs to the technical field of microbial vaccine preparation. Background technique [0002] Aeromonas hydrophila is widely distributed in nearshore seawater, rivers and lakes, and sewage wastewater. It is an opportunistic pathogen that can cause sepsis, diarrhea and other diseases in humans and various animals. Under certain conditions, it can cause outbreaks , In recent years, my country often has the report that because Aeromonas hydrophila causes many aquatic animals to fall ill or die in a large number (Lu Chengping, Acta Aquatica Sinica, 1992,16 (3)) and makes human being pathogenic. Brown flounder is one of precious economic fishes, and it is cultivated on a large scale in the northern coastal areas of China at present. The inventors have isolated pathogenic bacteria fro...

AI Technical Summary

Problems solved by technology

Brown flounder is one of precious economic fishes, and it is cultivated on a large scale in the northern coastal areas of China at present. The inventors have isolated pathogenic bacteria from Huida Aquatic Products Company in Tangshan, Hebei Province and Pulin Haizhen from brown flounder. Physiological, biochemical and molecular biological identifications showed that the bacterium was Aeromonas hydrophila, which was the direct pathogenic bacterium that caused ascites disease and sepsis in flounder, and diseased fish infected with this bacterium developed hydroperitoneal and intestinal hydrops And bleeding and other symptoms, the disease is highly contagious, and can lead to the death of a large number of flounder in severe cases, seriously endangering the development of the aquaculture industry

For many years, the prevention and treatment of this disease has mainly relied on the treatment of antibiotics. Although it has a certain effect, it is easy to produce drug resistance and cause drug residues in the body and in the river water environment, threatening the health and safety of the human body.