Method and kit for detecting Chinese holstein cattle lactoprotein ration property

Abstract

The invention discloses a method and a kit for detecting Chinese Holstein milk protein rate traits. The method to detect the milk protein rate of Chinese Holstein cows is to detect whether the deoxyribonucleotide of Chinese Holstein cattle from GenBank AccessionNo. , determine the genotype of the Chinese Holstein cattle, and then determine the milk protein rate trait by genotype. The kit of the present invention includes primers for PCR amplification of a genomic fragment containing nucleotides from the 873rd position from the 5' end (ie, the 279th position of GHR exon 8) from GenBank Accession No. NM_176608, restriction nucleic acid Endonuclease SSpI, PCR reagents other than the primers and restriction endonuclease buffer. The method and the kit of the invention are easy to operate, fast and sensitive, and the results detected by the method and the kit are reliable, stable and accurate. Therefore, the method and the kit of the present invention will have broad application prospects in the field of animal breeding.

Description

technical field [0001] The invention relates to a method and a kit for detecting the traits of Chinese Holstein milk protein rate. Background technique [0002] At present, the average yield per unit area of ​​dairy cows in major countries in the world can be divided into three levels: the United States, Canada, Japan, Israel and the European Union belong to the first level. The average yield per unit area of ​​dairy cows is above 6,000 kg. These countries mainly use intensive captive breeding; Australia, New Zealand, and Argentina belong to the second level. The average yield per unit of dairy cows is about 4,000 kilograms. These countries have large grasslands, and dairy cows are mainly raised on grazing, which has the advantage of low production costs; China, Brazil, Mexico and other countries belong to the third level. At different levels, the average yield per unit of dairy cows is less than 2,000 kg. It can be seen that the average yield level, breed structure and pro...

AI Technical Summary

Problems solved by technology

Therefore, the variation of the GHR gene structure may affect the structure and function of its expressed protein, thereby affecting the binding ability and signal transduction process of GH, that is, the mutation of the base sequence of the GHR gene may affect the normal function of GH, and ultimately affect the normal function of GH. Many traits such as milk production and meat production

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