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Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii

A technology of cryptodininosaurs and fatty acids, which is applied in the biological field, can solve the problems of simple content and no description of the extraction method of fatty acids in microalgae, and achieve the effects of simple operation, reduced extraction cost, and improved quality

Active Publication Date: 2010-04-07
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic patents mainly focus on the separation and extraction of long-chain polyunsaturated fatty acids from fish oil. There are only two patents related to the method of microalgae fermentation to produce long-chain polyunsaturated fatty acids (application numbers: 94106621.5 and 00135338.1), but the content is relatively simple , without a detailed description of the fatty acid extraction method from microalgae

Method used

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  • Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii
  • Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii
  • Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1 Fatty acid extraction and refining of algae harvested by fermentation in a 5L fermenter

[0053] Carry out 5 L tank fermentation according to above-mentioned cultivation method, harvest algae body. Pour the fermented liquid into the solvent tank, add 45g ferric chloride to make the cells flocculate and precipitate, after standing still, release the upper layer solution, add 800ml alcohol to the tank to make more bacteria settle, and release the upper layer liquid, The bacterial cells obtained above are centrifuged with a centrifuge to realize further solid-liquid separation. Use NaOH to prepare an aqueous solution with a pH of 14, pour the bacteria obtained in the previous step into the alkali wall, then send the cells into a colloid mill, grind the material into small particles, and send it to a homogenizer for high-pressure crushing to obtain Broken bacteria. Centrifuge again (speed 8000rpm) to remove water, pour the crushed thalline into the extraction ta...

Embodiment 2

[0056] Example 2 Fatty acid extraction and refining of algae harvested by fermentation in a 5L fermenter

[0057] Carry out 5 L tank fermentation according to above-mentioned cultivation method, harvest algae body. Pour the fermentation broth into a solvent tank, add 45g of aluminum sulfate to make the cells flocculate and precipitate, and after standing still, release the upper layer solution; add 800ml alcohol to the tank to make more bacteria settle, and release the upper layer liquid; Centrifuge the bacteria obtained above to realize further solid-liquid separation. Use NaOH to prepare an aqueous solution with a pH of 13, pour the bacteria obtained in the previous step into the alkali wall, then send the cells into a colloid mill, grind the material into small particles, and send it to a homogenizer for high-pressure crushing to obtain Broken bacteria. Centrifuge again (speed 8000rpm) to remove water, pour the broken bacteria into the extraction tank, pour it into the ex...

Embodiment 35

[0060] Example 35 The fatty acid extraction and refining of algae harvested by fermenting in a 35-ton fermenter

[0061] Carry out 5 ton tank fermentation according to above-mentioned culture method, harvest algae body. Pour the fermentation broth into a solvent tank, add 50kg of polyaluminum chloride to flocculate and precipitate the cells, and release the upper layer solution after standing still; add 15L alcohol to the tank to make more bacteria settle, and release the upper layer liquid as well; The bacterial cells obtained above are centrifuged with a centrifuge to realize further solid-liquid separation. Use NaOH to prepare an aqueous solution with a pH of 12, pour the bacteria obtained in the previous step into the alkali wall, then send the cells into a colloid mill, grind the material into small particles, and send it to a homogenizer for high-pressure crushing to obtain Broken bacteria. Centrifuge again (speed 8000rpm) to remove water, pour the broken bacteria into...

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Abstract

The invention discloses a process for extracting and refining fatty acid rich in DHA, from crypthecodinium cohnii, which comprises that first crypthecodinium cohnii fermentation liquor is flocculated,then solid and liquid are separated, walls are broken via alkali, and cells are mechanically broken, then the broken thalluses are extractsed via organic solvent, to obtain DHA rough oil, and the DHArough oil is degelatinized, alkali refined, decolorized, and deodorized to obtain DHA fine oil. The invention has simple operation and reduced extraction cost of DHA fat, which only uses organic solvent in operation, eliminates residue finally, and improves DHA fat quality by refining, while the DHA content in extractive is 40-50%, with DHA batch production support.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a process for extracting and refining fatty acid rich in DHA from Crypthecodinium cohnii. Background technique [0002] DHA (Docosahexaenoic acid, 22:6 △4.7.10.13.16.19 , full name docosahexaenoic acid) is an important long-chain polyunsaturated fatty acid (polyunsaturated fatty acid, referred to as PUFA), belonging to the n-3 series. As an essential component of cell membrane synthesis, DHA has the functions of enhancing eyesight and promoting the development of brain cells. It is an essential component for the growth and development of infant brains and the maintenance of normal brain functions for adults. It is known as "brain gold". DHA can also lower blood fat, prevent and treat arteriosclerosis, anti-inflammatory, anti-cancer and other effects. Therefore, DHA has received extensive attention from the food and medical circles. [0003] Traditional DHA production raw materials m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C57/03C07C51/42
Inventor 黄和任路静李霜胡南金明杰
Owner NANJING TECH UNIV
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