Fishes infectious spleen and kidney necrosis virus gene engineering vaccine and preparation method thereof

Abstract

The invention provides a genetic engineering vaccine of fish infectious spleen and kidney necrosis virus and a process for preparing the genetic engineering vaccine. The genetic engineering vaccine provided by the invention comprises two gene recombination proteantigens of protein-24 and protein-50, and the protein-24 and the protein-50 are cloned by two open reading frames of ORF24 and ORF50 which are screened from a genome of ISKNV, then are prepared recombinant plasmid, and are finished by expressing recombinant protein. The vaccine is a recombinant protein vaccine which is capable of inhibiting the infectious spleen and kidney necrosis virus from infecting the fish body, enabling the aquatic fish to grow healthily, thereby reducing the loss due to disease in the marine industry, increasing the output and benefit of the marine product, simultaneously avoiding drawbacks existing in DNA vaccine.

Description

technical field [0001] The invention belongs to the field of biological genetic engineering, and in particular relates to a fish infectious spleen and kidney necrosis virus (ISKNV) genetic engineering vaccine and a preparation method thereof. Background technique [0002] With the continuous development of my country's aquaculture industry, various aquaculture areas and aquaculture densities continue to increase. At the same time, improper management techniques in aquaculture make the aquaculture water environment deteriorating day by day, and various diseases frequently break out. Among the many diseases reported so far, one of the main causes of serious economic losses to fish farming is viral diseases. Among them, Infectious Spleen and Kidney Necrosis Virus (ISKNV, Infectious Spleen and Kidney Necrosis Virus) is one of the main viruses that cause a large number of fish deaths. [0003] The diameter of the mature virion of this virus is 150nm. Infected cells are swollen,...

AI Technical Summary

Problems solved by technology

In theory, the introduction of exogenous DNA into sensitive cells in the body may cause tumor cell formation by inserting and activating oncogenes, inserting and activating host cell proto-oncogenes or inserting and inactivating suppressor genes. Although this probability is very low; (2) exogenous Chronic expression of antigens may lead to adverse immunopathological responses; (3) use of genes encoding cytokines or co-stimulatory molecules may have additional hazards; (4) possible formation of antibodies against injected DNA and adverse autoimmune disorders; (5) The expressed antigen may produce unexpected biological activity

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