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Large scale insect pathogenic nematodes living high-power culturing method

A technology of entomopathogenic nematodes and living organisms, applied in protozoa, animal husbandry, etc., can solve the problem of reducing pathogenicity and achieve the effect of maintaining pathogenicity and prolonging lifespan

Inactive Publication Date: 2008-04-09
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology

Although the nematodes obtained by the traditional white trap method have high infectivity, their scale is far from meeting the needs of today's market. Guangdong Entomological Research Institute Han Richou adopts the method of artificial in vitro propagation. 1, 20 liters to the scale of Biosys Company to produce 15,000 liters of Steiner's nematodes and 7,500 liters of Heterobacter nematodes. Although the output of nematodes is high, its pathogenicity has decreased

Method used

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Embodiment Construction

[0016] The present invention is a part of the scientific and technological research project of Heilongjiang Provincial Science and Technology Department: "Research on Construction of Soil and Water Conservation Forest in Hilly Black Soil Area and Comprehensive Treatment of River Basin" (No. GA06B302-3). From March 2006 to June 2007, we carried out large-scale experimental production at the Erlongshan Ecological Experimental Station of the Heilongjiang Provincial Institute of Soil and Water Conservation Science according to the above steps. Three tree species were used for the test, namely, small black poplar and sylvestris sylvestris , Larch. All seedlings are 1-year-old seedlings. In the production, the best high-multiplication method of entomopathogenic nematodes was obtained, which can solve the problem that the number of nematodes is small and cannot be applied to production practice. After testing, the effect of preventing and controlling underground pests has reached mor...

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Abstract

The invention pertains to the technical field of forestry production, relating to a high cultivation method of entomopathogenic nematode in a large scale. The invention changes the present propagation in vitro into living propagation, which improves the pathogenic capability to pest in agriculture. The invention has high output and efficiency with low cost and good quality. The control effects are increased remarkably. The material used in the invention is widely distributed and inexpensive, which can reduce cost and be widely applied to the control towards hidden pest in forest, fruit trees, and ornamental gardens etc. and the underground pest in pot plants and cash crops. The invention can further improve the production quantity and quality of entomopathogenic nematode and promote the development of Biological control in our country.

Description

technical field [0001] The invention belongs to the technical field of forestry production. It is a large-scale high-power cultivation method for live entomopathogenic nematodes. Background technique [0002] Nowadays, chemical agents are mainly used in the control of underground pests and hidden pests, but this method is easy to cause poisoning of humans and animals, and also pollutes the environment. Moreover, the cost of repeated control is high, and the pests are prone to develop resistance. With the improvement of people's food safety requirements and the consideration of the potential crisis that chemical agents may bring to the ecological environment, it is urgent to screen biological control agents that can replace chemical agents. Today, Bacillus thuringiensis, Beauveria brongniatii, and Metarhizium anisopliae have been screened out as biological control agents, but the effect of controlling some underground pests and hidden pests is not very satisfactory. Entomo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10A01K67/033
Inventor 刘洪家王玉玺陈祥伟解运杰于泽波马光波
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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