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Fusarium oxysporum strain rejuvenation method

A Cuban-specialized, Fusarium oxysporum technology, applied in the field of agriculture-microbiology, to solve the contamination of miscellaneous bacteria, maintain the viability and pathogenicity, and achieve the effect of good controllability

Inactive Publication Date: 2018-11-23
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No other rejuvenation method with significant improvement has been reported so far

Method used

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  • Fusarium oxysporum strain rejuvenation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The 'Brazilian' banana (Musa AAA Cavendish subgroup) was used as the experimental material. Banana 'Brazil' is one of the most important cultivars for domestic banana production, and it is highly susceptible to race 4. According to the conventional methods and steps of shoot apex culture, rapid in vitro propagation of 'Brazil' banana was carried out, and rooted test-tube plantlets were obtained.

[0035] On this basis, a hydroponic system for 'Brazil' banana rooting test-tube plantlets was established with reference to the patent "A Banana Root System Isolated Hydroponic System and Cultivation Method" (publication number: CN107646661A), and hydroponic rooting test-tube plantlets were obtained.

[0036] The Foc No. 4 physiological race II5 bacterial strain that significantly declined in pathogenicity was used as the initial bacterial strain, and the spore suspension of the II5 bacterial strain (10 6 spores / mL) were prepared according to conventional methods.

[0037] U...

Embodiment 2

[0045] The Foc No. 4 physiological race ACC31282 with a significant decline in pathogenicity was used as the initial strain, and the spore suspension of the ACC31282 strain (10 6 spores / mL) were prepared according to conventional methods.

[0046] Under sterile conditions, the initial ACC31282 strain was reverted to 'Brazilian' banana hydroponic rooting test tube plantlets. When inoculating, 5mL of the spore suspension of the ACC31282 strain was directly added to the liquid medium of about 60mL of 'Brazilian' banana hydroponic rooting test-tube plantlets and shaken up to ensure that the pathogenic fungi fully contact the roots of the banana test-tube plantlets. After adding the spore suspension, the final concentration of spores in the liquid medium should reach 10 5 spores / mL.

[0047]3-4 weeks after the first backgrafting, observe the incidence of 'Brazilian' banana hydroponic rooting test-tube plantlets, and select the rooting test-tube plantlets with yellowed or withered...

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Abstract

The invention discloses a fusarium oxysporum strain rejuvenation method. Under sterile conditions, Foc strains with reduced pathogenicity are inoculated back into a sterile water culture rooting testtube seedling; then, the pathogenic bacterium separation is performed under the sterile conditions; the problem of sundry bacterium pollution is fundamentally solved; the surface sterilization link isomitted; after the separation culture, the bacterial colony does not need to be transferred or purified and can be directly used for spore suspension preparation; the operation steps are simplified.The method provided by the invention can be used for fast obtaining the rejuvenation Foc strains; the progress of the banana wilt disease resistance breeding work can be promoted.

Description

technical field [0001] The invention belongs to the field of agriculture-microbiology technology, and in particular relates to a method for rejuvenating the Fusarium oxysporum Cuban specialized strain. Background technique [0002] Fusarium oxysporum f.sp.cubense (Foc) is a soil-borne fungal disease caused by Fusarium oxysporumf.sp.cubense (Foc) infecting banana roots. At present, the disease has spread to all major banana producing countries in the world and has become one of the most devastating banana diseases. For a long time, Foc has been divided into four physiological races according to the pathogenicity difference of different banana strains or genera. Among them, race 4 has the strongest pathogenicity, and it can not only infect all cultivars susceptible to race 1 and race 2, but also infect the main cultivars for banana production in my country. Variety --- Banana (Musa AAA Cavendish subgroup). [0003] Research on the control of banana fusarium wilt has been car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/77
CPCC12N1/14C12N3/00
Inventor 吴元立黄秉智彭新湘杨护杨兴玉
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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