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Vaccine wine oil adjuvant containing momordica cochinchinensis soap and method for preparing the same

A technology of oil adjuvant and wood turtle, which is applied in the biological field, can solve the problems of weak immune response, poor preventive immune effect, and inability to effectively protect immunized animals, and achieve the effect of improving response ability and immune response intensity

Inactive Publication Date: 2008-04-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem that some existing oil adjuvant vaccines induce weak immune responses after injection into animals and cannot effectively protect the immunized animals
[0004] In order to overcome the shortcomings of some oil adjuvant vaccines that have poor preventive and immune effects on animal diseases, the present invention provides a new vaccine and its preparation. The vaccine prepared by using this formula can greatly improve the immune response intensity induced by the vaccine to animals. Enhance the ability of immune animals to resist infectious disease infection

Method used

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  • Vaccine wine oil adjuvant containing momordica cochinchinensis soap and method for preparing the same
  • Vaccine wine oil adjuvant containing momordica cochinchinensis soap and method for preparing the same
  • Vaccine wine oil adjuvant containing momordica cochinchinensis soap and method for preparing the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] The strengthening effect of example 1 cotyledon saponin (ECMS) and white oil combination to foot-and-mouth disease (FMD) vaccine immunity

[0027] 1. Materials and methods

[0028] 1. Experimental animals: 30 female DHP guinea pigs were purchased from the Experimental Animal Center of Zhejiang University.

[0029] 2. Antigen and adjuvant: the antigen is inactivated O-type foot-and-mouth disease virus (FMDV), provided by Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. White oil was purchased from Hangzhou Chemical Materials Co., Ltd. Cocaine saponins (ECMS) were prepared by our laboratory.

[0030]3. Vaccine preparation: First, dissolve the saponins of the plant with a small amount of normal saline, then mix it with the inactivated O-type foot-and-mouth disease virus (FMDV) antigen solution, then fully mix the mixture with an equal volume of white oil adjuvant to obtain final product.

[0031] 4. Immunization method: 30 guinea pigs wer...

example 2

[0063] Example 2 Test of piglets immunized with oil adjuvant foot-and-mouth disease vaccine containing different doses of saponins

[0064] 1. Materials and methods

[0065] 1. Experimental animals: 24 40-day-old piglets.

[0066] 2. Vaccine and adjuvant: Type O foot-and-mouth disease vaccine provided by Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences. Eucalyptus saponins (ECMS) saponins were prepared in this laboratory.

[0067] 3. Vaccine preparation: first dissolve the saponins of the fruit of the plant with a small amount of normal saline, then add the solution of the fruit of the plant to the vaccine and mix well, so that the content of the fruit of the plant per milliliter of the vaccine is 0 (control group), 0.5 , 1 and 2 mg.

[0068] 4. Immunization method: 24 piglets were randomly divided into 4 groups, 6 piglets in each group. Each pig was intramuscularly injected with 2ml of the vaccine once, blood was collected before and ...

example 3

[0072] Example 3 Contains the test of the oil adjuvant foot-and-mouth disease vaccine immunization piglets of the fruit saponin (4mg / dose)

[0073] 1. Materials and methods

[0074] 1. Experimental animals: 24 40-day-old piglets.

[0075] 2. Vaccine and adjuvant: Type O foot-and-mouth disease vaccine provided by Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences. Eucalyptus saponins (ECMS) saponins were prepared in this laboratory.

[0076] 3. Vaccine preparation: first dissolve the saponins of the fruit of the plant with a small amount of normal saline, then add the solution of the fruit of the plant to the vaccine and mix well, so that the contents of the fruit of the plant per milliliter of the vaccine are respectively 0 (as a control) and 2 mg.

[0077] 4. Immunization method: 24 piglets were randomly divided into 2 groups, 12 piglets in each group. Each pig was intramuscularly injected with 2ml of the vaccine once, blood was collect...

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Abstract

The invention relates to an oil-adjuvant vaccine with cochinchina momordica seed saponin and a method of preparing the vaccine. The vaccine comprises oil-adjuvant and cochinchina momordica seed saponin. The content of the cochinchina momordica seed saponin adjuvant in each milliliter of vaccine is 0.5 to 2.5 mg, and the oil-adjuvant accounts for 30 percent to 50 percent of the total volume of the vaccine. The preparation method is that: first the cochinchina momordica seed saponin is dissolved in a small amount of physiological saline and becomes solution; and then the cochinchina momordica seed saponin solution is mixed with antien; the mixture and the oil-adjuvant are fully emulsified and mixed with the help of emulsifier; or the oil-adjuvant and the cochinchina momordica seed saponin are emulsified and mixed, and then mixture is emulsified with the antien; or the cochinchina momordica seed saponin solution is added into the conventional oil-adjuvant vaccine to emulsify and mix. The animal immunization test with foot-and-mouth disease virus as the antien mode has shown, the oil-adjuvant vaccine with cochinchina momordica seed saponin of the invention can induce higher immune reaction strength than vaccine with single adjuvant.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the prevention of animal diseases, in particular to an oil adjuvant vaccine containing magnolia saponin and its preparation. The vaccine using the adjuvant can greatly improve the specific immune response intensity of animals. Background technique [0002] Infectious diseases are a class of important diseases that endanger animal health and cause serious economic losses to animal husbandry. Vaccination of animals is a routine method in many countries to prevent the occurrence of infectious diseases. Adjuvants can improve the immune effect of vaccines, and oil adjuvants are an important component of many animal infectious disease vaccines (such as foot-and-mouth disease vaccines). However, the immune effect of some commercial oil adjuvant vaccines is not very satisfactory. For example, the investigation by Xie Qin et al. showed that only 20.9% of piglets had a strong enough immune resp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P31/00A61P31/14A61K39/135
Inventor 胡松华肖琛闻
Owner ZHEJIANG UNIV
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