Preparation of aqueous extract and zymolyte of woodlousedry powder, and application of the same in medicine

A technology of ground beetle enzyme and water extraction, which is applied in the field of biomedicine, can solve the problems of weakening the therapeutic effect, large dosage, inconvenience for patients, etc., and achieve the effect of clinical application safety

Active Publication Date: 2008-04-02
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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AI Technical Summary

Problems solved by technology

[0003] The present invention is based on the in-hospital preparation of dry ground beetle powder in Jiangxi Provincial Dermatology Specialist Hospital, which shows good curative effect, quick effect, short treatment time and small side effects in the treatment of viral infectious skin diseases such as herpes zoster, chickenpox, herpes simplex and genital herpes. Advantages, aiming at the disadvantage of inconvenience caused by large dosage, the inventor speculates that the wood turtle and wood turtle are based on the protein properties of wood turtle and wood turtle dry powder, the property that protein can be degraded into polypeptides by pepsin after oral administration, and the good water solubility of polypeptides. Direct aqueous extracts and enzymatic hydrolyzates of dry powder have the potential to reduce the dosage without weakening the therapeutic effect

Method used

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  • Preparation of aqueous extract and zymolyte of woodlousedry powder, and application of the same in medicine
  • Preparation of aqueous extract and zymolyte of woodlousedry powder, and application of the same in medicine
  • Preparation of aqueous extract and zymolyte of woodlousedry powder, and application of the same in medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The preparation of embodiment 1 soil beetle water extraction polypeptide coarse powder

[0030] Suspend 15 g of dry wood beetle powder (obtained from grinding dried wood beetle) in 500 ml of distilled water. The resulting suspension was stirred at a constant temperature of 37.5°C for 40 minutes. The suspension was filtered naturally under normal pressure, and the filtrate was freeze-dried to obtain 2.25g (15%) of the ground beetle water-extracted polypeptide coarse powder (W 0 ), stored at 4°C until use.

Embodiment 2

[0031] Example 2 Separation and Purification of Water-extracted Polypeptide Coarse Powder of Wood Beetle

[0032] Sephadex G25 was processed and packed into a column (2.6×60cm), and 0.005mol / L ammonium acetate was filtered through a 0.45nm microporous membrane as a buffer balance. Dissolve 2.25g of ground beetle water-extracted polypeptide coarse powder in 1ml of distilled water, and centrifuge at 10,000 rpm for 10 minutes. The supernatant was applied to a Sephadex G25 chromatography column, eluted with 0.005mol / L ammonium acetate buffer solution, the flow rate was 0.5mL / min, and one tube was collected in 8 minutes. Each tube was measured by a UV spectrophotometer with a wavelength of 280nm, and the data was recorded. Curves were made with Microsoft Excel, fractions were pooled according to peak shape, and freeze-dried. yielded 0.24 g (10.5%) of W I , 0.54g (24%) W II , 0.74g (33%) W III and 0.19g (8.5%) W IV Four kinds of water-extracted peptide components.

[0033] Exa...

Embodiment 5

[0037] Example 5W IV The sequence of the polypeptide in

[0038] Measure W according to the method of Example 4 IV After the HPLC-ESI-MS, select the appropriate peak to measure the ESI-MS, analyze the collection of graphs (Fig. 4) according to the principle of complementarity, and obtain the amino acid sequence as follows: H-Ser-Cys-Leu(Ile)-Ser-Pro-Val-Ser -Leu(Ile)-Thr-Arg-Pro-Trp-OH.

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Abstract

The invention discloses a process of extracting an effective fraction for curing herpes zoster from ground beetle dry powder, and a series of polypeptide components obtained by the extracting process. The invention also discloses HPLC, Maldi-TOF and HPLC-ESI-MS finger prints and amino acid sequences of the polypeptide components, and application of the polypeptide components in curing herpes zoster. The extracting process of the invention includes the following procedures: water extract liquid and enzymolysis liquid of the ground beetle dry powder respectively receive chromatography by SephadexG25 column, to obtain a series of polypeptide components; and then the polypeptide components after separation are prepared into freeze-dry powder by the freeze-dry process. The clinical effect observation has shown the series of polypeptide components of the invention have obvious effect in curing the herpes zoster. The results of animal acute toxicity and sub-acute toxicity tests have shown, the polypeptide components of the invention are safe, without toxicity, and safe in clinical application.

Description

technical field [0001] The present invention relates to the preparation process of the effective part of Chinese medicinal materials, especially to the preparation process of the effective part of the dry ground turtle powder. The present invention also relates to the related polypeptide isolated from the effective part, its amino acid sequence, its fingerprint and their use in the treatment of herpes zoster The application belongs to the field of biomedicine. Background technique [0002] Shingles is a common skin disorder that not only affects large numbers of people, but can also be fatal in some severe cases. In the early 1980s, Jiangxi Provincial Dermatology Specialist Hospital accidentally discovered Eupolyphaga Seu Steleophaga during the investigation of skin diseases; aliases: Eupolyphaga Seu Steleophaga; Alias: Eupolyphaga, Eupolyphaga, Crossing Street, Ground Turtle, Knotbug, Bedbug Mother; Origin: Hunan, Hubei , Jiangsu) has a clear effect on herpes zoster. Sinc...

Claims

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Application Information

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IPC IPC(8): A61K38/10A61K38/08A61K35/64A61P31/22C12P21/02
Inventor 彭师奇赵明魏蕾曹连甲邵丹耿承芳叶发舜廖银根
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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