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Stichopus japonicus step-by-step hybrid breeding method

A technology of crossbreeding and sea cucumber, which is applied in the field of progressive crossbreeding, can solve problems such as growth vigor, resistance to stress, and yield decline, asynchronous gonad development, and indistinguishable appearance, etc., to achieve strong activity, many spines, and growth speed fast effect

Inactive Publication Date: 2008-03-26
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the seed dominance of Chinese-Russian hybrid sea cucumbers is generally only shown in the F1 generation, and the traits segregate from the self-crossed F2 generation. The individual differences in the F2 generation group are very large, the growth is irregular, and the growth vigor and stress resistance are not uniform. Compared with the F1 generation, the sex and yield were significantly lower than that of the F1 generation, so that the dominance of the F1 generation declined gradually; at the same time, the selfing of the F1 generation would inevitably lead to the homozygosity of alleles and the recessive and harmful traits. The F2 generation and subsequent generations of ginseng selfing are generally no longer used in production.
[0006] Although the theory and practice of genetic breeding have proved that the breeding of progressive hybridization (also known as modified hybridization and absorption hybridization) has the advantages of improving the stress resistance and vitality of the cultured objects, but due to the asynchronous development of the gonads of the improved sea cucumber in China In addition, sea cucumbers are dioecious, and it is difficult to distinguish them from the appearance, so the current progressive hybridization is limited to mammals (meat dogs, sheep, cattle, etc.), and there is no stepwise hybridization of sea cucumbers in existing publications. any reports of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. Take Chinese-Russian hybrid sea cucumbers, put them into indoor breeding ponds in November of that year, cultivate them at a constant temperature of 10-12°C, and carry out indoor ripening, and start to heat up in April of the following year, increasing the temperature by 1°C every day, and then raising the temperature to 14°C; , the Chinese-Russian hybrid sea cucumbers were cultured in pre-set cages in outdoor ponds, with a density of 1-2 heads / m 2 ;From mid-May, sampling and dissecting the gonads, when the egg diameter reaches 120μm, put the Chinese-Russian hybrid sea cucumber back into the indoor breeding pond, and cultivate at a constant temperature of 16-17°C; when the Chinese-Russian hybrid sea cucumber re-enters the indoor breeding pond , take Chinese sea cucumbers and put them into another indoor breeding pond, and cultivate them at a constant temperature of 16-17°C; during the cultivation period, feed them in a conventional way;

[0023] b. Sampling and disse...

Embodiment 2

[0031] The basic steps are the same as in Example 1, except that in step a, it is better to feed the mixed liquid of sargassum grinding solution and sea mud during the first indoor cultivation period, and the weight percentages of sargassum grinding solution and sea mud are 33% respectively , 67%. Sea mud is net mud, which refers to floating mud on fishing boats hanging nets. This mud is rich in organic matter, which is very conducive to the growth of sea cucumbers. It is also possible to add defatted fish meal and shrimp feed into the mixed liquid, and the weight of the defatted fish meal and shrimp feed does not exceed 10% of the mixed liquid.

Embodiment 3

[0033] The basic steps are the same as in Example 2, except that the second indoor constant temperature cultivation of step a and the indoor constant temperature cultivation of Chinese sea cucumber are fed with a mixture of egg yolk and spirulina and filtered with a 200-mesh net. The weight percentages of egg yolk and spirulina are respectively 90%, 10%, 5% of the body weight of the pro-ginseng is fed every day.

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Abstract

The present invention discloses a stichopus japonicus grading breeding method. It is characterized by that said method includes the following steps: making sex glands of Chinese stichopus japonicus and Chinese-Russian hybrid stichopus japonicus be simultaneously developed and matured, using shade-drying parturifacient method to respectively obtain sperm of Chinese stichopus japonicus and ovum of Chinese-Russian hybrid stichopus japonicus, making artificial insemination and incubating fertilized ovum until the laval plankton is formed, after the laval plankton is attached, utilizing conventional method to breed F1 generation of stichopus japonicus of Chinese stichopus japonicus and Chinese-Russian stichopus japonicus, then using said F1 generation stichopus japonicus as seed stichopus japonicus, accelerating the sex glands of said F1 generation stichopus japonicus and Chinese stichopus japonicus to make them be synchronously matured, making artificial insemination and breeding so as to obtain a new young of stichopus japonicus.

Description

Technical field: [0001] The invention relates to a sea cucumber breeding method, in particular to a stepwise hybrid breeding method using Chinese sea cucumbers and Chinese-Russian hybrid sea cucumbers as parents. Background technique: [0002] Apostichopus japonicus is a famous and valuable seafood, rich in protein and viscous polysaccharides, with high nutritional health and medicinal value, popular with consumers, high price, and large market capacity. In recent years, with the gradual expansion of the consumption demand of sea cucumbers, the scale of artificial cultivation of sea cucumbers has become larger and larger, and the cultivation methods are various. [0003] In the past, sea cucumber seedlings were mainly obtained by harvesting wild seedlings or artificial seedlings. Among them, artificial seedlings were the main source. After natural maturation or artificial maturation, artificial fertilization and incubation were carried out to cultivate larvae, immature and j...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A23K1/18A23K50/80
CPCY02A40/81
Inventor 常亚青江曙光韩波堂宋坚张玉勇
Owner DALIAN OCEAN UNIV
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