White spirit flavor enhancer and application method thereof in wine production
A flavoring agent and liquor technology, applied in the field of liquor flavoring and wine making, can solve the problems of high value, no liquor flavoring, etc., and achieve the effects of eliminating harshness, rich taste, and facilitating preservation and transportation.
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Embodiment 1
[0023] Materials: Refined lard, purchased from Shenzhen Simin Oil Trading Co., Ltd., Aspergillus niger lipase, a commercially available product produced by Shenzhen Lvweikang Bioengineering Co., Ltd., soybean lecithin, from Shanghai Xirun Chemical Industry Co., Ltd.
[0024] Reagent: 0.2 mol / L potassium phosphate buffer pH 8.0.
[0025] 1.0mol / L NaOH
[0026] step:
[0027] Weigh 1 kg of refined lard with a balance, measure 2 L of 0.2 mol / L potassium phosphate buffer solution (pH8.0) with a graduated cylinder, add it to the reaction vessel, and add 200,000 units of Aspergillus niger lipase, with an enzyme activity of 20,000 U / L In terms of g, 10.0 grams of Aspergillus niger lipase needs to be added, and the pH value is adjusted to 7.0 with sodium hydroxide solution, and reacted for 10 hours at 40° C. During the reaction, the stirrer in the reaction tank is constantly stirred, and the stirring speed is 400 rpm. Obtain the lipase hydrolysis product of Aspergillus niger lard, i...
Embodiment 2
[0029] Materials: Refined lard, purchased from Shenzhen Simin Oil Trading Co., Ltd., Aspergillus niger lipase, a commercially available product produced by Shenzhen Lvweikang Bioengineering Co., Ltd., soybean lecithin, from Shanghai Xirun Chemical Industry Co., Ltd.
[0030] Reagent: 0.2mol / L potassium phosphate buffer pH 8.0.
[0031] 1.0mol / L NaOH
[0032] step:
[0033] Weigh 1 kg of refined lard with a balance, measure 2 L of 0.2 mol / L potassium phosphate buffer solution (pH8.0) with a graduated cylinder, add it to the reaction vessel, and add 500,000 units of Aspergillus niger lipase to obtain an enzyme activity of 20,000 U / L In terms of g, 25.0 grams of Aspergillus niger lipase needs to be added, and the pH value is adjusted to 7.0 with sodium hydroxide solution, and reacted at 40° C. for 8 hours. During the reaction, the stirrer in the reaction tank is constantly stirred, and the stirring speed is 400 rpm. Obtain the lipase hydrolysis product of Aspergillus niger lard...
Embodiment 3
[0035] Materials: soybean oil, purchased from Shenzhen Simin Oil Trading Co., Ltd., rhizopus lipase, purchased from Novozymes, and sorbitan fatty acid ester, purchased from Shanghai Xirun Chemical Industry Co., Ltd.
[0036] Reagent: 0.2mol / L sodium phosphate buffer pH 8.0.
[0037] 1.0mol / L NaOH
[0038] step:
[0039] Take 1 kilogram of soybean oil with a balance, measure 1.5 L of 0.2 mol / L sodium phosphate buffer solution (pH8.0) with a graduated cylinder, join in the reaction vessel, and add 100,000 units of rhizopus lipase, and use the enzyme activity of 20000U / G meter, need add 5.0 gram rhizopus lipases, adjust pH value to 7.0 with sodium hydroxide solution, 40 ℃ of reactions 8 hours, in the reaction process, keep stirring with the stirrer in the reaction tank, stirring speed 300 rev / mins, Obtain the lipase hydrolysis product of O. sojae, and inactivate the lipase at 100° C. for half an hour. A novel liquor flavoring agent was obtained by adding 0.1 gram of sorbitan ...
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