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Method for preparing water-soluble beta-1,3/1,6-dextran

A dextran and water-soluble technology, which is applied in the field of water-soluble β-1, can solve the problems of low yield and achieve the effects of improving purity and yield, reducing environmental pollution, and reducing costs

Inactive Publication Date: 2008-02-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can produce water-soluble β-glucan that is relatively pure and has better biological activity, but the yield is low (total sugar content 85%), and removal of chitin, protein, fat and nucleic acid etc. is not enough (chitin 4.5%, the rest are between 1 and 5%)

Method used

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  • Method for preparing water-soluble beta-1,3/1,6-dextran
  • Method for preparing water-soluble beta-1,3/1,6-dextran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, preparation of water-soluble β-1,3 / 1,6-glucan

[0039] 1. Preparation of water-insoluble β-1,3 / 1,6-glucan

[0040] The production process of water-insoluble β-1,3 / 1,6-glucan is shown in Figure 1, and the specific steps are as follows:

[0041] 1. Enzymatic hydrolysis of yeast cell wall: 100g of yeast cell wall (the product was purchased from Hubei Yichang Angel Yeast Co., Ltd., "Fubon" brand), 100mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml Mix with water (mass ratio: 1000:1:5000), and stir with a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory, Huma brand adjustable speed stirrer, 200-300 rpm) under the condition of 37°C, and the stirring time is After 30 minutes, centrifuge at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge, the same below) for 20 minutes to remove the supernatant to obtain 95.3 g of precipitate, which is the product of yeast cell wall enzymatic hyd...

Embodiment 2

[0062] Embodiment 2, preparation of water-soluble β-1,3 / 1,6-glucan

[0063] 1. Preparation of water-insoluble β-1,3 / 1,6-glucan

[0064] The production process of water-insoluble β-1,3 / 1,6-glucan is shown in Figure 1, and the specific steps are as follows:

[0065] 1. Enzymatic hydrolysis of yeast cell walls: Mix 200g of yeast cell walls, 400mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml of water, and use a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory) at 35°C , Huma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 60 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 25 minutes. Cleared to obtain 191.20g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0066] 2. Alkali treatment: 191.20 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall is added to 2000 ml of 0.5 M Na...

Embodiment 3

[0086] Embodiment 3, preparation of water-soluble β-1,3 / 1,6-glucan

[0087] 1. Preparation of water-insoluble β-1,3 / 1,6-glucan

[0088] The production process of water-insoluble β-1,3 / 1,6-glucan is shown in Figure 1, and the specific steps are as follows:

[0089] 1. Enzymatic hydrolysis of yeast cell walls: Mix 200g of yeast cell walls, 600mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 1000ml of water, and use a constant speed stirrer at a medium speed at 40°C (Shanghai Specimen Model Factory) , Puma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 30 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 20 minutes. Cleared to obtain 190.8g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0090] 2. Alkali treatment: 190.8 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall was added to 2000 ml of 1.0 M Na...

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Abstract

The invention discloses a method for preparing water-soluble beta-1, 3 / 1, 6-qlucan. The method has the steps as follow: 1) oxidization process: suspending water-insoluble beta-1, 3 / 1, 6-qlucan in oxidant solution at 2 to 8 DEG C, laying aside for oxidizing reaction for 16 to 24 hours for centrifugation and collection; 2) washing: stirring and washing the deposition treated from step 1) by ethyl alcohol and acetone respectively for 30mins to 1 hour for then centrifugation and collection; 3) ultrasonic process: heavy-density suspending the deposition treated from step 2) in the DMSO solution of concentration expressed in percentage by volume of 0.05 to 0.2 percent, and ultrasonic processing the heavy-density suspension liquid for 20 mins; and centrifugally collecting the supernatant fluid to achieve water-soluble beta-1, 3 / 1, 6-qlucan. Being detected, the water-soluble beta-1, 3 / 1, 6-qlucan prepared by the method has the yield over 95 percent and has little protein content and fat content.

Description

technical field [0001] The invention relates to a preparation method of water-soluble β-1,3 / 1,6-glucan. Background technique [0002] Yeast is a single-celled fungus and is the most widely used and economically valuable microorganism so far. The international FDA recognizes brewer's yeast as a safe microorganism and has been widely used in the production of beverages and foods. At present, my country consumes about 2,000 tons of yeast annually and produces about 3 tons of waste yeast sludge. Since the yeast cell wall is the main by-product of the beer manufacturing industry, and the main treatment method for it today is to sell it cheaply as roughage or discharge it directly, it causes a lot of waste of resources and pollution to the environment. [0003] Several research results have shown that pure yeast cell walls have obvious immune-boosting effects on animals, and also exhibit growth-promoting effects. Further evidence shows that the main mechanism is that the β-1,3 / ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12P19/04
Inventor 呙于明张博
Owner CHINA AGRI UNIV
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