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Mammary cancer immunodeficiency type animal model and method for preparing the same

An immunodeficient mouse and breast cancer technology, applied in medical science, surgery, diagnosis, etc., to achieve the effect of short construction period, low animal mortality, and good repeatability

Active Publication Date: 2008-01-30
GENOR BIOPHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many reports on the successful reconstruction of the human immune system and transplantation of human tumors using Nod-SCID mice, there have been no reports on the transplantation model of MCF-7 breast cancer cells in Nod-SCID mice at home and abroad so far.

Method used

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  • Mammary cancer immunodeficiency type animal model and method for preparing the same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of breast cancer Nod-SCID mouse model

[0030] Source of material:

[0031] MCF-7 breast cancer cells: ATCC serial number is: HTB-22

[0032] Nod-SCID mice: purchased from Shanghai Slack Experimental Animal Co., Ltd., certificate number: SCXK (Shanghai) 2003-0003. Preparation:

[0033] 1. Preparation of estradiol cyvalproate sterile injection:

[0034] Weigh 6mg of estradiol cypionate, add 2 drops of absolute ethanol, 2 drops of soil temperature 80, grind for 10 minutes, add normal saline to make the volume to 20ml, that is, the concentration is 0.3mg / ml, and then perform autoclaving for 10 minute.

[0035] 2. Preparation of MCF-7 breast cancer cells:

[0036] Cultivate MCF-7 breast cancer cells in vitro, collect the well-grown MCF-7 cells in vitro, wash them with PBS three times, and adjust the cell fluid density to 5×10 7 piece / m1.

[0037] 3. Breeding and selection of Nod-SCID mice:

[0038] There were 8 Nod-SCID mice without immune leak...

Embodiment 2

[0045] The preparation of embodiment 2 breast cancer Nod-SCID mouse model

[0046] Test conditions: estradiol valerate was selected as estradiol, MCF-7 breast cancer cell fluid density was 5×10 6 Individual / ml, all the other preparation conditions are the same as embodiment 1.

[0047] Test results: 10 days after inoculation, tumors can be observed to grow subcutaneously in mice. And with the growth of culture time, the tumor continued to grow.

Embodiment 3

[0048] The preparation of embodiment 3 breast cancer Nod-SCID mouse model

[0049] Test conditions: Ethinyl estradiol cyclopentyl ether is selected as estradiol, MCF-7 breast cancer cell liquid density is 5×10 8 Individual / ml, all the other preparation conditions are the same as embodiment 1.

[0050] Test results: 10 days after inoculation, tumors can be observed to grow subcutaneously in mice. And with the growth of culture time, the tumor continued to grow.

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Abstract

The present invention relates to the technical field of medical evaluation and detection. The invention discloses a method to prepare a breast cancer immunodeficiency mouse model, which establishes a human breast cancer cell Nod-SCID mouse model firstly and successfully at home and abroad by regular injection of estrogen preparations to the immunodeficiency mice and transplant of the breast cancer cells after thereof. The breast cancer immunodeficiency mouse model constructed by the invention has short construction period, high tumor formation rate, low animal mortality, simple and easy construction method and low cost, and thus is an ideal breast cancer immunotherapy animal model.

Description

technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to an immunodeficiency animal model of breast cancer and a preparation method thereof. Background technique [0002] Among malignant tumors of the female reproductive system, breast cancer accounts for about 7% to 10% of all malignant tumors in the whole body. The incidence of breast cancer is on the rise all over the world. No matter in high-incidence areas or low-incidence areas, the incidence of breast cancer is increasing at a rate of 5-20%. [0003] Animal models of breast cancer are widely used in anti-breast cancer drug screening and pharmacodynamic research, and many literatures have reported the application of existing breast cancer animal models in anti-breast cancer drug screening and pharmacodynamic research. The production of breast cancer animal models plays a key role in the study of the etiology and pathogenesis of breast cancer, so as to ef...

Claims

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Application Information

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IPC IPC(8): A61B19/00A61B5/00A01K67/027
Inventor 王小晨刘全海
Owner GENOR BIOPHARMA
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