Method of preparing edible gelatin with combined zyme
A technology combining enzymes and gelatin, which is applied in food preparation, application, food science, etc., can solve problems such as incomplete degradation of bone gelatin protein, increased cost, and impact on gelatin quality, so as to reduce subsequent processes and unstable factors, and reduce environmental pollution. Pollution, the effect of shortening the production cycle
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Embodiment 1
[0021] Embodiment 1, the bone collagen (bone particle 30 ~ 50mm, pH 4 ~ 5, water content of bone bone 62.3%) 5.44kg after adopting the traditional alkaline process to pickle and wash the bone collagen is subjected to a combined enzymatic hydrolysis experiment:
[0022] Add three times the amount of osteosin and soak in water for 2 hours to expand the osteosin and remove oil, add pepsin with a quality of 2.65‰ of osteosin, raise the temperature to 60°C, and perform the first enzymatic hydrolysis; after 2 hours of enzymatic hydrolysis, heat up rapidly To 85°C, maintain the high temperature for 30 minutes to inactivate the enzyme, then adjust the pH of the reaction solution to 6-7 with sodium hydroxide, extract the gelatin at 60°C for 2 hours, consume 60-80% of the bone mass, and estimate the gelation rate to be 51 ~68%.
[0023] Add three times the volume of water to the remaining bone bone, and adjust the pH of the system to about 6 with sodium hydroxide; then add AS1.398 neutr...
Embodiment 2
[0026] Example 2, 100 kg of bone collagen (bone grains 10-20 mm, pH 4-5) washed with pickling and water by the traditional alkaline process was added to 100 kg of water 3 times the volume of bone bone and soaked for 3 hours to make the bone bone swell; After degreasing, add pepsin with an amount of 1.5‰ osteosin, raise the temperature to 60°C, and carry out the first enzymatic hydrolysis; Calcium hydroxide adjusts the pH of the reaction solution to 6-7, and then extracts the gel at 60°C for 2 hours. After the gel is dried, the gel yield of the first enzymatic gelatin is 30-35%, the viscosity is 2.5mPa·s, and the freezing force is 30-35%. 189 Bloom g.
[0027] Add water three times the volume of the remaining bone mass, adjust the pH of the system to 7-8 with calcium hydroxide, add 2701 alkaline protease with 1‰ of the original dry bone mass for the second enzymatic hydrolysis: enzymatic hydrolysis at a temperature of 60°C for 2 hours Afterwards, rapidly raise the temperature ...
Embodiment 3
[0029] Example 3: Take the 10-20mm bone grains after pickling and water washing in the traditional alkaline process, add 100kg of bone bone with a pH of 4-5, add about 2 times the volume of bone bone and soak for 3 hours, and add cathepsin with a dry bone mass of 1.5‰ , raise the temperature to 60°C, and carry out the first enzymolysis; after 3 hours of enzymolysis, rapidly raise the temperature to 90°C, maintain the high temperature for 20 minutes to inactivate the enzyme, and then adjust the pH of the reaction solution to 6-7 with sodium hydroxide; then Extract the gelatin at 60°C for 2 hours. After the gelatin is dried, the gelatin yield of the first enzymolysis gelatin is 30-35%, the viscosity is 2.5mPa·s, and the freezing force is 189Bloom g;
[0030] Add water 2.5 times the volume of the remaining bone mass, use sodium hydroxide to adjust the pH of the system to 7-8, add trypsin with 2‰ of the original dry bone mass, and carry out the second enzymatic hydrolysis at 60°C, ...
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