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Technique for preparing gingkgo extractive with low content of ginkgolic acid

A technology of Ginkgo biloba extract and Ginkgo phenolic acid is applied in the field of extraction of natural plant active components, and can solve problems such as increased production cost and cost, and achieve the effects of solving temperature limitation, reducing production cost and shortening production cycle.

Inactive Publication Date: 2007-12-26
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing patent application No. 03114826.3 mentions the method of preparing low ginkgolic acid content, but in this preparation method, when extracting, concentrating, diluting and settling, it needs to be carried out under the condition of -1 ° C ~ 20 ° C, so that In the general situation of production, cooling system needs to be added, thereby increasing the cost; The more expensive acid-removing resin DAD-1 increases the production cost and brings additional development costs for the acid-removing resin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 25 kg of ginkgo leaves, use 10, 10, and 8 times the amount (solvent volume to medicinal material weight, the same for the following) 60% ethanol, stir and extract three times at 70 ° C for 2 h, 2 h, and 1 h respectively, release the extract, combine and concentrate To 1.5 times the volume of the medicinal material, add 2 times the volume of purified water, adjust the pH value to 4.5-5.0 with dilute hydrochloric acid, stir evenly, and after standing for 30 minutes, centrifuge in a three-legged centrifuge to obtain a centrate, which is passed through AB-8 Type macroporous adsorption resin adsorption, eluted with 20% ethanol and 80% ethanol in turn, collected 80% ethanol eluate, concentrated under reduced pressure, at a temperature of 50-60°C, concentrated to about 0.4 times the volume of the eluent, and passed through AB -8 type macroporous adsorption resin for adsorption, eluting with 70% ethanol, concentrating the eluent under reduced pressure, and spray drying to o...

Embodiment 2

[0022] Take 50kg of ginkgo leaves, use 12 and 10 times the amount of 70% ethanol, stir and extract twice at 80°C for 2 hours and 2 hours respectively, release the extract, combine and concentrate to 2.0 times the volume of the medicinal material, add 2 times the volume of purified water , use 20% sulfuric acid to adjust the pH to 1.0-2.0, stir evenly, and centrifuge in a high-speed centrifuge to obtain a centrifuge, which is absorbed by D101 macroporous adsorption resin, eluted with 15% ethanol and 60% ethanol in turn, and collected 60% ethanol eluate, concentrated under reduced pressure, temperature 50-60°C, concentrated to about 0.5 times the volume of the eluent, then passed through D101 macroporous adsorption resin, eluted with 70% ethanol, and the eluate was concentrated under reduced pressure, Spray-dried to obtain 1.36kg of ginkgo extract with low ginkgolic acid content. The total flavonoids of ginkgo is 30.8%, ginkgolide is 12.1%, and ginkgolic acid is 3.4ppm.

Embodiment 3

[0024] Take 200kg of ginkgo leaves, use 12 and 10 times the amount of 80% ethanol, stir and extract twice at 80°C for 2 hours and 2 hours respectively, release the extract, combine and concentrate to 1.6 times the volume of the medicinal material, add 3 times the volume of purified water , adjust the pH to 3.0-3.5 with 10% citric acid, stir evenly, and filter through plate and frame to obtain the filtrate. The filtrate is absorbed by D101 macroporous adsorption resin, eluted with 10% ethanol and 80% ethanol in turn, and collected 80% ethanol eluate, concentrated under reduced pressure at a temperature of 45-55°C, concentrated to about 0.8 times the volume of the eluent, added an equal volume of petroleum ether (60-90°C) for extraction, and then added 0.5 times the volume of petroleum ether to the lower layer (60-90° C.) extraction, taking the lower layer, concentrating under reduced pressure, and spray-drying to obtain 6.0 kg of Ginkgo biloba extract with low ginkgolic acid con...

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PUM

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Abstract

The present invention relates to an extraction technique of natural plant active component. In the concrete, it relates to a preparation process of ginkgo extract with low ginkgolic acid content. In the ginkgo extract obtained by adopting the invented preparation method the ginkgo total flavone content is 28-40%, bilobalide content is 6-14% and ginkgolic acid content is less than 5ppm. Its preparation method includes the steps of extraction, separation, purification, concentration and drying.

Description

technical field [0001] The invention relates to the extraction technology of natural plant active ingredients, in particular to a simple preparation process of ginkgo extract (GBE) with low ginkgolic acid content. Background technique [0002] The existing patent application No. 03114826.3 mentions the method of preparing low ginkgolic acid content, but in this preparation method, when extracting, concentrating, diluting and settling, it needs to be carried out under the condition of -1 ° C ~ 20 ° C, so that In the general situation of production, a cooling system needs to be added, thereby increasing the cost; on the other hand, in order to remove the ginkgolic acid, the content of the ginkgolic acid in the finished product is lower than 5ppm. The more expensive acid-removing resin DAD-1 not only increases the production cost, but also brings additional development costs for the acid-removing resin. Contents of the invention [0003] For overcoming above-mentioned weak p...

Claims

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Application Information

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IPC IPC(8): A61K36/16C07B63/00C07H1/08
Inventor 袁干军刘军保陈志斌涂蓉黄红谦苏秋玲
Owner HAINAN MEDICAL COLLEGE
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