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Method for identifying codonopsis pilosula and preparation containing codonopsis pilosula

A technology of Codonopsis pilosula and preparations, applied in the field of quality control of pharmaceutical preparations, can solve the problems of complicated operation, incomplete application of preparations, rare reference substances, etc., and achieve the effects of easy availability of materials, clear spots and low prices

Inactive Publication Date: 2007-12-12
TIANJIN TASLY ZHIJIAO PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is applicable to the medicinal materials of Codonopsis pilosula, but it is not fully applicable to the preparations containing Codonopsis pilosula; and, the C used in this method 18 Solid-phase extraction cartridges are expensive, and reference substances are also rare; in addition, this method is cumbersome to operate and difficult to master

Method used

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  • Method for identifying codonopsis pilosula and preparation containing codonopsis pilosula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take Shenqi Fuzheng freeze-dried powder (prepared according to the method of Chinese patent application CN 1546152A Example 1), add methanol 25ml, ultrasonic treatment for 30min, filter, the filtrate is concentrated to dryness, the residue is dissolved in 10ml of water, and added to the treated AB -8 resin column (resin bed volume 12ml, resin loading height about 11cm), washed with water until colorless, then eluted with 10ml 50% ethanol, finally eluted with 15ml 95% ethanol, collected 95% ethanol eluate , evaporated to dryness, and the residue was dissolved in 1ml of absolute ethanol as the test solution. Take Codonopsis reference medicinal material 2g, and make Codonopsis reference medicinal material solution by the same method. According to the thin-layer chromatography (Appendix VI B of Chinese Pharmacopoeia 2005 edition), absorb 5-10 μl of the test solution and the reference medicinal material solution, respectively spot on the same silica gel G plate, and mix with...

Embodiment 2

[0020] The test product is Shenqi Wuweizi Tablets (the first part of "Chinese Pharmacopoeia" edition in 2005), according to the method of Example 1, the difference is that the resin used is D 101 type macroporous adsorption resin. In the chromatogram of the test product, at least one fluorescent spot of the same color is displayed at the position corresponding to the chromatogram of the control medicinal material.

Embodiment 3

[0022] Test product is Weishuning granules (the first part of "Chinese Pharmacopoeia" edition in 2005), according to the method operation of embodiment 1, difference is that used resin is X AB-8 type macroporous resin. In the chromatogram of the test product, at least one fluorescent spot of the same color is displayed at the position corresponding to the chromatogram of the control medicinal material.

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Abstract

The invention provides a medicinal herb of radix codonopsis and a method for discriminating preparation containing radix codonopsitis. The method comprises the following steps in sequence: preparation of testing solution for assay: a preparing medicinal herb of radix codonopsitis, extract of radix codonopsitis, or preparation containing radix codonopsitis into aqueous solution, loading on macroporous adsorbent resin column, eluting with 30-70& lower alcohol and 75-100% lower alcohol in sequence, collecting eluant of 75-100% lower alcohol, concentrating to dry, and dissolving in lower alcohol as testing solution for assay; b preparing contrast medicinal herb of radix codonopsitis into contrast solution according to the preparation method of testing solution for assay; c sucking testing solution for assay and contrast solution, dotting on the same silica gel g plate, developing with n-butanol-glacial acetic acid-water of ratio of 1-10:1:0.2-7 as developer, taking out, air-drying, and detecting with ultraviolet lamp; wherein steps a and b has no precedence order. The inventive method has simple operation and low cost.

Description

technical field [0001] The invention relates to a quality control method of a Chinese medicinal material and a pharmaceutical preparation containing the medicinal material, in particular to a quality control method of the Chinese medicinal material Codonopsis pilosula and a pharmaceutical preparation containing Codonopsis pilosula or Codonopsis extract. Background technique [0002] Codonopsis pilosula, Codonopsis pilosulaNannf., Codonopsis pilosula Nannf.Var.modesta L.T.Shen, Codonopsis tangshen Oliv., Codonopsis tubulosaKomar. Codonopsis subglobosa (W.W. Smith) and the root of Codonopsis canescens Nannf. are sweet in taste and flat in nature, and belong to the spleen and lung meridians. They are traditional Chinese medicines for invigorating Qi. At present, a considerable part of the Chinese patent medicines and herbal medicines that have been marketed at home and abroad contain Codonopsis or Codonopsis extracts, so it is very important to quickly and accurately identify C...

Claims

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Application Information

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IPC IPC(8): A61K36/344G01N30/90
Inventor 夏忠庭叶正良郑永锋李学敏
Owner TIANJIN TASLY ZHIJIAO PHARMA
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