Application of ophiopognin saponin D in preparing medicine for regulating vascular endothelial cells apoptosis gene

A technology of vascular endothelium and Ophiopogon japonicus saponin, which is applied in the application field of Ophiopogon japonicus D in the preparation of gene medicines regulating the vascular endothelial cell cycle, which can solve the problems of low sensitivity, interference peaks, and inability to use

Inactive Publication Date: 2011-06-01
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0009] Because saponin is a kind of compound with large polarity and complex structure, it has no ultraviolet absorption and no specific chromogenic agent, and the determination of Ophiopogon japonicus saponin is often interfered by sugar. If the terminal absorption method of ultraviolet detection is used, the sensitivity Low, with interference peaks, unusable

Method used

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  • Application of ophiopognin saponin D in preparing medicine for regulating vascular endothelial cells apoptosis gene
  • Application of ophiopognin saponin D in preparing medicine for regulating vascular endothelial cells apoptosis gene
  • Application of ophiopognin saponin D in preparing medicine for regulating vascular endothelial cells apoptosis gene

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Embodiment

[0016] Example: Research on Ophioside D Regulating Apoptotic Genes of Vascular Endothelial Cells

[0017] The effective component of Radix Ophiopogon japonicus saponin D extracted from the Chinese herbal medicine Ophiopogon japonicus has anti-H effect on human umbilical vein endothelial cells. 2 o 2 effect on apoptosis. The present invention utilizes the gene chip technology to discover the vascular endothelial cell gene expression profile regulated by Ophiopogon japonicus saponin D for the genes related to cell apoptosis.

[0018] 1 Materials and methods

[0019] 1.1 Main reagents: RPMI-1640 medium (GIBCO, USA) (containing 20% ​​calf serum per liter, L-glutamine 0.33mg, penicillin 100u, streptomycin 100u, pH-7.4); digestive agent 0.25% Trypsin (MERCK, USA); Radix Ophiopogon japonicus. (L.f.) Ker-Gawl. produced in Mianyang, Sichuan, purchased from Nanjing Medicinal Materials Company, identified as Ophiopogon japonicus. (L.f.) Ker-Gawl.

[0020] RNA extraction reagents: R...

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Abstract

The invention uses a cellular apoptosis gene chip for detecting H2O2 and specific expression of 120 pieces of ophiopogon saponin D controlled key apoptosis gene. Research with gene chip technology shows that the H2O2 can remarkably up regulate 27 pieces of gene and down regulate 3 pieces of gene while the ophiopogon saponin D performs antagonism to the above gene, thereby protecting vascular endothelial cell, which is importance part for preventing and treating cardio-cerebrovascular diseases.

Description

1. Technical field [0001] The invention relates to the new application of Ophiopogonis saponin D in pharmacy, in particular to the application of Ophiopogonis saponin D in the preparation of gene medicines regulating the cycle of vascular endothelial cells, which belongs to the technical field of traditional Chinese medicine. 2. Background technology [0002] 1. Ophiopogon japonicus completes the effect of nourishing yin and promoting blood circulation by protecting vascular endothelial cells [0003] Ophiopogon japonicus is a commonly used product for nourishing yin liquid clinically, and it is one of the representative drugs for nourishing yin and promoting body fluid. Ophiogon japonicus, the dried tuber root of Ophiogon japonicus (Thumb.) Ker-Gawl. It mainly contains a variety of glycosides: Ophioside B, D, 23, 24 dihydroxy Rosko soap, Ophioside-3-O-a-L-rhamnopyranosyl (1-2)-β-D-pyridine Glucopyranoside, methyl philodendron flavanone A, B, ophiopogon flavanone A, B, 6-f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P9/00C12Q1/68C07H1/08
Inventor 张旭陆兔林蔡宝昌王明艳毛春芹张小燕张志洁杨进
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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