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Methods for scavenging oxidizing nitrogen and oxygen species with fragrances having antioxidative properties

A scavenger, aromatic ring technology, used in gasification substances, formulations of perfume preparations, cosmetics, etc.

Active Publication Date: 2007-12-05
ACCESS BUSINESS GRP INT LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The scavenger can comprise a fragrance or a fragrance ingredient or the scavenger can be a fragrance

Method used

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  • Methods for scavenging oxidizing nitrogen and oxygen species with fragrances having antioxidative properties
  • Methods for scavenging oxidizing nitrogen and oxygen species with fragrances having antioxidative properties
  • Methods for scavenging oxidizing nitrogen and oxygen species with fragrances having antioxidative properties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1: DPPH spectrophotometer analysis method

[0081] The free radical 2,2-diphenyl-1-picrylhydrazly (DPPH) can be used to determine the free radical scavenging ability of a test material such as a scavenger of the present invention. Table 2 reports the amount of various scavengers needed to consume 50% of the DPPH starting material (EC 50 ). Measurements were taken after the reaction of the scavenger being tested and DPPH had reached a steady state. DPPH are free radicals that react with antioxidants to change color. The color change of DPPH is an indication of the antioxidant capacity. Antioxidant capacity is defined as 1 / EC 50 , where EC 50 is the concentration of antioxidant required to reduce the color of DPPH by 50%. Therefore, DPPH serves as a model for the oxidative properties of free radicals. Provided below are the details for operating the DPPH spectrophotometer analysis:

[0082] Examples of scavenger tests:

[0083] - Weigh 100mg of sample ...

Embodiment 2

[0093] Example 2: Nitric Oxide Radical Absorbance Ratio (NORA) Analysis

[0094] The NORA assay is the determination of compounds that quench NO * Nitric oxide free radicals (NO * ) capabilities. In the analysis, target compounds are oxidized using the nitric oxide generator NOR-3, resulting in increased fluorescence. EC 50 . Provided below are the details for performing the Nitric Oxide Radical Absorbance Ratio (NORA) analysis:

[0095] Reagent:

[0096] Dihydrorhodamine 123, Molecular Probes (Cat * D632)

[0097] NOR-3 ((±)-(E)-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamine),

[0098] Calbiochem (#489530)

[0099] Phosphate Buffer (Certified pH 7.00), Fisher (Cat#SB108-1)

[0100] N,N-Dimethylformamide, Fisher (Cat#D119)

[0101] DMSO Fisher (Cat#D136)

[0102] Methanol, HPLC pure.

[0103] Reagent preparation:

[0104] DHR123: 1.0mg / 3ml MeOH=1mM raw material (frozen and protected from light)

[0105] 7.5 μL stock solution / 10 ml buffer = 0.75 μM working solut...

Embodiment 3

[0131] Example 3: Scanning analysis of volatile compounds

[0132] The volatility of compounds can be determined using capillary gas chromatography with flame ionization detector (GC-FID) as described below:

[0133] 1. Place approximately 1 mg of scavenger test compound or sample into a 1.5 mL glass autosampler vial fitted with a screw cap with a Teflon threaded septum. Tighten the screw cap and allow the sample to equilibrate at room temperature for 2 hours. Water was used as blank control.

[0134] 2. Place the sample vial into the autosampler of a gas chromatograph (GC) equipped with a capillary column, splitless injection, and a flame ionization detector (FID). Set the GC conditions according to the following GC methods:

[0135] GC: HP6890 or equivalent

[0136] Column: DB-I (J&W Scientific size is 25m×0.32mm film thickness 25μm) Carrier gas: Helium linear velocity is 35cm / sec (~12.4psi, 1.5mL / min)

[0137] Intake condition:

[0138] Injection: splitless

[0139] ...

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PUM

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Abstract

Described herein are methods and compositions for scavenging reactive molecules in the air, including free radicals, reactive oxygen and reactive nitrogen species. In particular, described herein are methods and compositions for scavenging airborne active molecules prior to contact of the active compounds with skin and other tissue surfaces. The scavenging effect is produced by topical application of the scavenging agent, such as a volatile antioxidant, to the skin, or by releasing the scavenging agent, such as a volatile antioxidant, into the air near or in close proximity to the skin. In addition, the present invention relates to compositions comprising a combination of a scavenger and a carrier or excipient, wherein the scavenger comprises a fragrance or fragrance ingredient having antioxidant properties, and the scavenger scavenges the air surrounding the skin site to which the composition is applied active molecules in. The compositions of the present invention are also used in household products such as candles, deodorants and pet litter to scavenge indoor reactive oxygen and nitrogen species.

Description

[0001] This application claims priority to US Provisional Application Serial No. 60 / 633,752, filed December 7, 2004, the entire contents of which are incorporated herein by reference. field of invention [0002] The present invention relates to compositions and methods for scavenging reactive molecules from the air including free radicals, reactive oxygen and reactive nitrogen species. More particularly, the present invention relates to methods and compositions for scavenging airborne active molecules before these active molecules come into contact with the skin, eye, mucous membrane, or other tissue surface. Acts by topical application of scavengers, such as volatile antioxidants, to the skin, including around or near the eyes and mucous membranes, or release of scavengers, such as volatile antioxidants, into the skin, eyes, or mucous membranes nearby and in the surrounding air. In addition, the present invention relates to a composition comprising a scavenging agent and its...

Claims

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Application Information

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IPC IPC(8): A61K8/34A61Q17/00A61K8/35A61L9/04A61K8/97
CPCA61K8/922A61K8/22A61K8/40A61K8/97A61K2800/522A61L9/04A61L9/044A61Q1/02A61Q13/00A61Q17/00A61Q19/00A61K8/9789A61K8/9794
Inventor J·C·莱弗里特J·R·迈恩S·R·米斯勒J·V·希梅卡R·C·罗思
Owner ACCESS BUSINESS GRP INT LLC
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