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Spectrophotometric measurement in color-based biochemical and immunologigal assays

A content and colored technology, applied in biochemical equipment and methods, analytical materials, biological testing, etc., can solve problems such as background characteristics and color effects

Inactive Publication Date: 2007-12-05
IMI INT MEDICAL IINNOVATIONS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] A persistent problem with the known mucus test is the need for visual inspection and evaluation of the staining results
This method is susceptible to the properties and color of the background (i.e. skin)

Method used

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  • Spectrophotometric measurement in color-based biochemical and immunologigal assays
  • Spectrophotometric measurement in color-based biochemical and immunologigal assays
  • Spectrophotometric measurement in color-based biochemical and immunologigal assays

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Embodiment Construction

[0050] As stated above, Applicants' basic invention, namely the measurement of chromaticity and / or hue angle using colorimetric measurements, in particular color-based analysis, can find application in many ways and examples. These two aspects of the invention and some preferred embodiments are described in detail below, separated by each heading.

[0051] Spectrophotometers suitable for use in all aspects of the present invention are portable, reflectance-based, and allow color characteristics (such as hue angle, luminosity, and Hue or Saturation) accurate measurement. Such instruments are commercially available. A preferred example of a suitable instrument is "Spectrophotometer Model CA22" from X-Rite, Grand Falls, Michigan, U.S.A. Appropriate software accompanying it can be linked to a computer to give an accurate reading of the hue angle of the dyed sample tested. The spectrophotometer accepts reflections in the wavelength range of about 400-700 nm, ie most of the visib...

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PUM

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Abstract

A process is provided for analyzing a specimen of biological material in any of a number of biochemical or immunological tests for an analyte which involves subjecting the specimen to treatment which develops a color correlating to the amount of analyte in the specimen. According to the invention at least one defined color characteristic selected from hue angle, chroma, saturation and lightness of the developed color is measured and the results of that measurement analyzed to determine the presence or concentration of the analyte in the specimen. Particular applications are to the detection of cancerous or pre-cancerous abnormalities from the analysis of lung mucus, throat mucus, cervical mucus or seminal fluid.

Description

[0001] The patent application of the present invention is PCT / CA 00 / 00918 with the international application date of August 4, 2000, and the application number of entering the Chinese national phase is 00813497.9, and the name is "color-based biochemical and immunological analysis. The divisional application of the invention patent application of "spectral measurement". technical field [0002] The present invention relates to color-based biochemical and immunological assays and tests in which analytical or test samples are subjected to spectroscopic measurements of color properties, in particular hue angle and / or chromaticity. Analysis using such measurements has been demonstrated to produce quantitative or semi-quantitative results in a wide range of drug testing and screening procedures and diagnostic methods. Background technique [0003] As described in detail below, many diagnostic tests rely on visual inspection and the evaluation of a color that is specific to the an...

Claims

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Application Information

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IPC IPC(8): G01N33/92G01N33/52G01N33/574A61B5/00A61K49/00C12Q1/60
Inventor M・J・伊夫利
Owner IMI INT MEDICAL IINNOVATIONS INC
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