ELISA method for determining sudan 1 in food
An enzyme-linked immunosorbent assay and analysis method technology, applied in the field of enzyme-linked immunosorbent assay, to achieve the effects of high sensitivity, low test cost, and simple sample processing
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[0041] 1. Preparation of modified Sudan Red 1
[0042] Sudan Red 1 is a small molecular compound, which has no immunogenicity and cannot directly immunize animals to produce antibodies. The molecular structure of Sudan Red 1 must be chemically modified reasonably and effectively so that it has an active group so that it can be combined with the carrier protein Cross-linking to produce immunogens and coating antigens.
[0043] Preparation of two kinds of Sudan Red 1 modifications: p-aminophenylpropionic acid was oxidized by nitrite and then reacted with 2-naphthol in ice-salt bath to synthesize Sudan Red 1-3-propionic acid (Sudan 1-C3) ; P-aminobenzenebutyric acid reacted with 2-naphthol under the same conditions to synthesize Sudan Red No. 1-4-butyric acid (Sudan 1-C4).
[0044] (1) Preparation of Sudan 1-3-propionic acid (Sudan 1-C3)
[0045] Step 1: Dissolve 0.02-0.08mol of malonic acid in 20-80mL of freshly steamed pyridine, add 0.02-0.1mol of p-nitrocinnamic acid and 0.0...
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