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ELISA method for determining sudan 1 in food

An enzyme-linked immunosorbent assay and analysis method technology, applied in the field of enzyme-linked immunosorbent assay, to achieve the effects of high sensitivity, low test cost, and simple sample processing

Inactive Publication Date: 2007-11-28
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Enzyme-linked immunosorbent assay (ELISA) has the advantages of high sensitivity, strong specificity, and rapid analysis. ELISA has been used in the analysis fields of medical clinics, drugs, food and the environment, but there is no ELISA determination of food in the world. Literature Reports on the Content of Sudan Red No.1

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  • ELISA method for determining sudan 1 in food
  • ELISA method for determining sudan 1 in food
  • ELISA method for determining sudan 1 in food

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Experimental program
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Embodiment

[0041] 1. Preparation of modified Sudan Red 1

[0042] Sudan Red 1 is a small molecular compound, which has no immunogenicity and cannot directly immunize animals to produce antibodies. The molecular structure of Sudan Red 1 must be chemically modified reasonably and effectively so that it has an active group so that it can be combined with the carrier protein Cross-linking to produce immunogens and coating antigens.

[0043] Preparation of two kinds of Sudan Red 1 modifications: p-aminophenylpropionic acid was oxidized by nitrite and then reacted with 2-naphthol in ice-salt bath to synthesize Sudan Red 1-3-propionic acid (Sudan 1-C3) ; P-aminobenzenebutyric acid reacted with 2-naphthol under the same conditions to synthesize Sudan Red No. 1-4-butyric acid (Sudan 1-C4).

[0044] (1) Preparation of Sudan 1-3-propionic acid (Sudan 1-C3)

[0045] Step 1: Dissolve 0.02-0.08mol of malonic acid in 20-80mL of freshly steamed pyridine, add 0.02-0.1mol of p-nitrocinnamic acid and 0.0...

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Abstract

The invention discloses an ELISA analysis method for content of sudan red No.1 in food. It is characterized in that two sudan red derivatives with different carbon bridge lengths are compounded and derivatives are crosslinked with carrier protein to obtain four polyclonal antibodies. There are eight test combinations. Seven combinations can be used for immunoassay to sudan red No.1. Concentration range of standard curve is 0.1-100ng / mL and IC50 is 0.3-2.0ng / mL. Cross reaction rate of four antibodies and sudan red No.2-4 is 0.1-14.3%. There is almost no cross reaction between four antibodies and six pigments added in food. It shows that antibodies have very high specificity to sudan red No.1. Six commercial foods can be used as samples. After it is treated simply, extract liquid is diluted directly for ELISA measurement. The recovery of standard addition for sudan red No.1 is 92.4-114% and relative standard deviation is 5.9-24.8%. Samples with standard addition are analyzed by HPLC and are contrasted with ELISA results. Two methods have higher relativity (R=0.9851; n=7).

Description

technical field [0001] The invention relates to an enzyme-linked immunosorbent assay method (ELISA) for measuring the content of Sudan Red No. 1 in food, belonging to the research field of food safety supervision or food analysis. Background technique [0002] Sudan red is a kind of artificially synthesized azo oil-soluble chemical dyeing agent, which is widely used in industrial products such as motor oil, automobile wax and shoe polish, and can also be used in the coloring of fireworks and fireworks. Household red floor wax or red shoe polish usually contains Sudan Red No. 1. With the gradual understanding of the toxicity of Sudan Red, the International Agency for Research on Cancer classifies Sudan Red as a third-class carcinogen, which is potentially dangerous. [0003] In the process of food processing, in order to improve the sensory properties of food and increase people's appetite, some food coloring is often added in order to make the color of the product bright or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/535
Inventor 邓安平韩丹
Owner SICHUAN UNIV
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