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Nitro-reductive pseudomonads and its use

A technology of Pseudomonas and nitro, which is applied in the field of nitroreducing Pseudomonas, can solve the problems of refractory biodegradation and effective degradation of benzothiazoles, etc.

Active Publication Date: 2007-11-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, benzothiazole is a difficult biodegradable organic compound, and it is difficult for traditional treatment methods to completely and effectively degrade benzothiazole in a short period of time and achieve ideal results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1, the isolation and cultivation of P.nitroreducens S1 bacterial strain

[0016] 1. Isolation of strains

[0017] Sludge samples were collected at the sewage treatment plant of Fushun Petroleum No. 3 Plant. Take 1 drop of sludge aqueous solution on the screening medium, add 0.1g of benzothiazole, spread it evenly with a spreader, put it in an incubator, and cultivate it at 30°C for 2 days; use an inoculation loop to transfer the screened strains to LB The culture medium is placed in an incubator at 30° C. for 1 day, and the obtained strain is the strain that degrades benzothiazole.

[0018] Described screening culture medium component is:

[0019] (NH 4 ) 2 SO 4 5g; K 2 HPO 4 1g;

[0020] K H 2 PO 4 4g; NaCl 2g;

[0021] MgSO 4 ·7H 2 O 1g; Agar 15g;

[0022] Water 1000mL; pH 7.0.

[0023] 2. Culture of strains

[0024] Use an inoculation loop to inoculate the isolated strains into shake flasks with a liquid volume of 5...

Embodiment 2

[0031] The cultivation of P.nitroreducens S1 strain is the same as that in Example 1. Take 2 shake flasks, add 50mL sterile water and 0.6mL benzothiazole respectively. One of the shake flasks was taken as a blank control group, and the cultured S1 bacterial agent was added to the other shake flask to make its OD value 4.8. The two shaking flasks were placed on a shaker to shake the reaction, and the conditions of the shaker were: temperature 30° C.; rotation speed 150 rpm. After 10 hours, samples were taken and analyzed, and the results are listed in Table 2.

[0032] initial

Embodiment 3

[0034] The cultivation of P.nitroreducens S1 strain is the same as that in Example 1. Take 2 shake flasks, fill them with 50mL sterile water, and then add 4g of soil contaminated by 0.6mL benzothiazole. Take one shake flask as a blank control group; add S1 bacterial agent to the other shake flask to make its OD value 5.0. Place 2 shake flasks on a shaker to shake the reaction. Shaking table conditions are: temperature 30°C; rotation speed 150 rpm. After 10 hours, samples were taken for analysis, and the results are listed in Table 3.

[0035] initial

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PUM

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Abstract

The Pseudomonas nitroreducens S1 is prepared by separating out from active sludge in sewage disposal tank of petrochemical plant, purifying and culturing. The preservative number is CGMCC No. 1488. It has excellent efficient degradable performance for benzothiazole and can be used to dispose waste water and waste gas containing benzothiazole, polluted water and soil.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of nitroreductor pseudomonas and its application. Background technique [0002] Heterocyclic compounds are important fine chemical raw materials. Due to their unique chemical structure, they show unique properties different from other traditional benzene-based and naphthalene-based fine chemical intermediates. They are widely used in dyes, medicines, pesticides, additives, etc. field. At present, compounds with heterocyclic structures have become hot development products and development trends in the field of fine chemicals. The production and application of heterocyclic compounds will produce a lot of "three wastes", especially the large amount of waste water. With the rapid development of domestic heterocyclic compounds, the treatment of "three wastes" containing heterocyclic compounds is increasingly necessary and urgent. [0003] Benzothiazole is one such ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84B09C1/10C12R1/38
CPCY02A50/20
Inventor 范峰佟明友黎元生许谦
Owner CHINA PETROLEUM & CHEM CORP
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