Composite enzyme prepn for degrading agricultural waste effectively and its prepn process
A technology for compound enzyme preparation and agricultural waste is applied in the field of high-efficiency degradation of agricultural waste compound enzyme preparation and its preparation, which can solve the problems of low fermentation temperature, insufficient fermentation, incomplete killing of harmful organisms, etc. The effect of biogas production, energy saving, and promotion of digestion and absorption rate
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[0013] A compound enzyme preparation for efficient degradation of agricultural waste, which includes the following enzyme species and activities: xylanase, whose enzyme activity is ≥100,000 U / g; β-glucanase, whose activity is ≥80,000 U / g ; Cellulase, whose enzyme activity is ≥28,000 U / g; acid protease, whose enzyme activity is ≥56,000 U / g; lipase, whose enzyme activity is ≥15,000 U / g pectinase, whose enzyme activity is ≥0.6 million U / g; α-amylase, whose enzyme activity is ≥12,000 U / g.
[0014] The preparation method of the high-efficiency degradation compound enzyme preparation of the above-mentioned agricultural waste, the method comprises the following process steps:
[0015] 1. Strain preparation:
[0016] Xylanase, glucanase, cellulase, acid protease, lipase, pectinase, α-amylase, etc. are all used in vitro directed evolution of enzyme molecules to produce high-yield enzyme live strains.
[0017] Using the theory of molecular breeding, using the directional screening tec...
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