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Pearl sacs assistant formation method after nucleus-insertion

An auxiliary molding and pearl capsule technology, applied in biochemical equipment and methods, climate change adaptation, microorganisms, etc., can solve the problems of difficult wounds, lack of high-quality beads, and inability to carry out large-scale promotion, so as to improve the quality of pearls and reduce the formation of rate, and the effect of shortening the time for the formation of cell capsules

Inactive Publication Date: 2007-09-19
ZHEJIANG GRACE PEARL JEWELRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the wound at the insertion site is difficult to heal well, most of the pearls formed are inferior pearls such as tail pearls and black pearls, and rarely become high-quality pearls, so large-scale promotion operations cannot be carried out.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Cut out the front mantle tissue of a robust 3-year-old high-quality hybrid Hydrangea sinensis;

[0021] (2) Under the condition of temperature of 20 degrees and pH value of 6.9, the mantle tissue was enzymolyzed with trypsin for 40 minutes, and the cell tissue was obtained by dispersing;

[0022] (3) Using high-speed centrifuge equipment to separate the cell tissue obtained in step (2) to obtain cell fluid;

[0023] (4) neutralize the resulting cell fluid;

[0024] (5) Weigh 40 g of glucose, 10 g of monosialytetrahexanoic acid ganglioside, and 0.8 g of norfloxacin to prepare 1 L of protection solution, soak the neutralized cell solution in the obtained protection solution for 20 minutes for later use;

[0025] (6) Take 0.1ml of the cell solution obtained in step (5), and drop it on the postoperative wound of the pearl oyster after the nucleation operation, the cell solution will fuse with the cell pieces and the bead nucleus to form a pearl sac.

Embodiment 2

[0027] Pearl sac-assisted forming steps are the same as in Example 1, and the environmental parameters, the raw material components of the protective solution and the consumption of the cell fluid are as follows:

[0028] Temperature: 21 degrees

[0029] pH: 7.1

[0030] Glucose: 60g

[0031] Monosialytetrahexanoic acid ganglioside: 15g

[0032] Norfloxacin: 1.2g

[0033] Enzyme hydrolysis time: 38 minutes

[0034] Soaking time: 22 minutes

[0035] The amount of cell fluid: 0.1ml.

Embodiment 3

[0037] Pearl sac-assisted forming steps are the same as in Example 1, and the environmental parameters, the composition ratio of the protective solution and the consumption of the cell solution are as follows:

[0038] Temperature: 22 degrees

[0039] pH: 7.2

[0040] Glucose: 40g

[0041] Monosialytetrahexanoic acid ganglioside: 15g

[0042] Norfloxacin: 1.2g

[0043] Enzyme hydrolysis time: 35 minutes

[0044] Soaking time: 22 minutes

[0045] The amount of cell fluid: 0.15ml.

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PUM

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Abstract

The present invention discloses a pearl sac assistant forming method after transplanting nuleus, which comprise the following steps: (1) cutting front segment mantle tissue of pearl oyster (2) using protease for enzymolysis of mantle tissue under temperature 20-25 degree, pH value 6.9-8.3, dispersing the tissue and obtaining cell (3) separating the obtained cell in step (2) by high speed centrifugal apparatus, cell fluid is obtained; (4) neutralizing the cell liquor, (5) immersing neutralized cell fluid in protection liquid; (4) taking optimum volume of cell liquor, dropping it at the wound of transplant operation. The cell liquid integrated with mantle and pearl nucleus to form pearl sac. The present invention successfully operate the cell engineering method in pearl cultivation that cell sac forming time is shortened 10 days, pearl quality is obviously promoted as compared to normal pearl breeding method which largely reduce the formation rate of abnormal pearl and nude pearl without pearl layer.

Description

technical field [0001] The invention relates to the technical field of pearl cultivation, in particular to a pearl sac auxiliary forming method after pearl core insertion. Background technique [0002] Freshwater pearls are generally cultivated as non-nuclear pearls. The method adopted is to insert small pieces of cells into the mantle of river mussels. The pearls produced by this method are called non-nuclear pearls. The breeding cycle of non-nuclear pearls is long, the diameter of the produced pearls is small, and the economic benefits are also low. [0003] At present, existing freshwater nucleated pearls are cultivated for reference from the seawater pearl cultivation method, and the method adopted is to directly send the pearl nucleus into the clam mantle to cultivate pearls. Because the wound at the insertion site is difficult to heal well, most of the pearls formed are inferior pearls such as tail pearls and black pearls, and rarely become high-quality pearls so that...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12N5/06C12N5/07
CPCY02A40/81
Inventor 张高亮詹伟建邱咸王坚郑蒋江邵伟焕张康
Owner ZHEJIANG GRACE PEARL JEWELRY
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