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External regenerating and breeding water lettuce

A kind of water-floating lotus body and water-floating lotus technology, which is applied in the field of plant tissue culture and achieves the effects of good genetic stability, broad application value and rapid growth.

Inactive Publication Date: 2007-08-29
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Problems solved by technology

Since this method does not go through the callus induction stage, the cultivated sterile vaccine can only be used for biochemical research, and this method cannot be used for genetic transformation

Method used

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  • External regenerating and breeding water lettuce

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Embodiment Construction

[0021] The present invention establishes a method for in vitro regeneration of a brand-new water floating lotus that induces callus through the stem of water floating lotus, regenerates seedlings through callus, and uses liquid medium to expand and propagate. This method will be widely used in both basic and applied research.

[0022] The invention provides a method for in vitro regeneration of water lotus, which comprises the following steps:

[0023] a. Disinfection of explants: select the stems of the water plank lotus, wash and disinfect them, and obtain sterile explants. The disinfection steps are as follows: first, treat in 5% sodium hypochlorite disinfectant solution for 10 minutes, then transfer to 0.1% mercuric chloride solution for disinfection for 3 minutes, and then wash with sterile water for 3 times.

[0024] b. callus induction: stems are cut into thin slices, and then placed in a mixture containing plant hormone 2,4-dichlorophenoxyacetic acid (2,4-dichlorophen...

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Abstract

The present invention relates to a method for external regeneration and propagation method of pistia stratiotes. Said method includes the following steps: selecting pistia stratiotes stem, cleaning and disinfecting it to obtain sterile explant; cutting its stem into slices, then placing the slice on the MS culture medium containing plant hormone 2,4-D and BA, inducing to form callus; then transferring said callus onto the MS culture medium containing plant hormone BA to make regeneration and obtain seedling, further rooting so as to obtain whole regenerated plant, then transferring the regenerated plant onto SH liquid culture medium to make extensive propagation.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for in vitro regeneration and propagation of water floating lotus obtained through in vitro aseptic culture through callus induction. Background technique [0002] Water lotus (Pistia stratiotes L.), also known as Dapiao, is a plant of the Araceae family, a floating aquatic plant, and a different family of plants from water hyacinth (Eichhornia crassipes). Water lily is considered to be the fastest growing aquatic plant in the world. One plant can asexually reproduce 250,000 plants within 90 days. It has good genetic stability and is non-toxic to the human body. The main biomass of water floating lotus is leaves, which are rich in protein, carotene, total flavonoids and trace elements, among which essential amino acids account for about 50%. It can not only be used as feed and fertilizer, but also has strong anti-pollution ability, and can purify pollutants such as N...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 陈士云张勇杨宝玉王瑶王友如
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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