Purity testing method for tomato hybrid based on PCR technology

Abstract

This invention relates to one tomato mixture purification test method based on PCR technique in biological technique field, which comprises the following steps: using product mixture tomato Shu power 8 as subject to extend molecule label filtering to identify one RAPD and one SSR label object of local abnormal band; RAPD label generate mother property label of NAURP409800 and father property label of NAURP4091100; SSR label lead object property mother label of NAUSSRTOM47140 and father label of NAUSSRTOM 47148.

Description

technical field [0001] The invention relates to a method for detecting the purity of tomato hybrids based on PCR technology. 1 Identification of genetic purity of seeds of hybrid variety 'Sufen 8'. Belongs to the field of biotechnology. Background technique [0002] Tomato (Lycopersicon esculentum.L.) is one of the important vegetable crops in the world, with a planting area of ​​about 450,000 hectares in 2005 and an annual output of more than 120 million metric tons (FAO, http: / / www.fao.org / ). Since tomato is a self-pollinated plant, in the process of hybrid seed production, if artificial detasseling is not timely, false hybrids will often appear, resulting in a decrease in the genetic purity of the seeds, causing huge economic losses to production, and at the same time bringing losses to the seed supply unit. to the great risk of claims. Therefore, the excellent F 1 Rapid and accurate identification of the genetic purity of generation hybrid seeds and seedlings has bec...

AI Technical Summary

Problems solved by technology

This method is time-consuming, labor-intensive, takes up a large amount of land, and needs to go through a growth and maturity period of 3-5 months; sometimes the number of morphological characters that can be used for hybrid identification is limited, and most morphological characters are easily affected by environmental factors, so only The accuracy of identifying the purity of varieties by morphological characteristics will be affected

The isoenzymes used for the purity identification of tomato varieties mainly include POD, EST, ACP, ADH, MDH, etc. However, due to the small genetic variation and low polymorphism of cultivated tomato, there are few types of available enzymes, and their expression and tissue It is related to the development period, and it is difficult to use some F 1 Hybrid purity testing and variety identification

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