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Thrombolytic medicine synergist and its preparing process

A technology for thrombolytic drugs and synergists, applied in the field of biopharmaceuticals, can solve the problems of patent applications and literature reports that no synergists are found, and achieve the effects of improving thrombolytic effects, reducing usage, and reducing drug costs.

Inactive Publication Date: 2007-08-15
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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AI Technical Summary

Problems solved by technology

[0004] So far, no patent applications and literature reports about P11 as a synergist of thrombolytic drugs have been found

Method used

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  • Thrombolytic medicine synergist and its preparing process
  • Thrombolytic medicine synergist and its preparing process

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Embodiment 1

[0038] (1) Cloning of human P11 protein gene and construction, screening and identification of prokaryotic expression plasmid

[0039] Strains and expression vectors: the expression strains Escherichia coli BL21, E.coli DH5α, and plasmid pBV220 are all preserved by the Institute of Basic Medical Sciences, Academy of Military Medical Sciences of the Chinese People's Liberation Army. They are all publicly available elsewhere.

[0040] Enzymes and biochemical reagents: restriction enzymes BamHI, EcoR I, Taq DNA polymerase and T 4 DNA ligase was purchased from TaKaRa Company; nucleic acid molecular weight standard DL2000 was purchased from TaKaRa Company; protein molecular weight standard was purchased from Jingke Bioengineering Company; DNA purification kit was from Boda Biotechnology Company; plasmid extraction kit was from Promega Company. The Bradford method protein quantification kit was purchased from Pleibo Company. Human thrombin, human plasminogen, human fibrinogen, and...

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Abstract

The invention discloses a synergist for thrombolysis and its preparation method, which primarily adopts widely available P11protein as synergist for thrombolysis and other medicine. The synergist can enhance the activity of drugs for thrombolysis, improve thrombolysis effect thereby reducing medicamentous consumption and relieve adverse side effect, and reducing cost.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a thrombolytic drug synergist and a preparation method. Background technique [0002] P11 protein (calpactinI, light chain) is a member of the S100 family (Schafer BW et al., Trends Biochem Sci, 1996, 21(4): 134-140), also known as S100A10, is a polypeptide containing 97 amino acid residues (11Kda). P1 1 protein has an EF-hand type structure and exists in the form of helix-loop-helix (S. Rety, et al., Nat. Struct. Biol. 6 (1999) 89-95.27). P11 protein usually regulates the blood fibrinolytic system (G.Kassam, B etc., Biochemistry37 (1998) 16958-16966.) as the subunit of annexin (annexinII) heterotetramer complex (AIIt), can be alone or with membrane Annexin II stimulates tissue-type plasminogen activity factor (t-PA) in the form of a complex to regulate plasminogen activity (Travis J. et al., Biological Chemistry, 2003, 278 (28): 25577-25584 ). In the human body,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61P7/02C12P21/02C12N15/12A61K38/17
Inventor 徐东刚姚广印邹民吉王旻房涛
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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