Formula constituted by cytokine and compound, its action of promoting nerve regeneration and action of researching and diagnosing nervous system disease

A cytokine, nerve regeneration technology, applied in diagnosis and treatment, prescription in the field of nervous system disease research

Inactive Publication Date: 2007-08-08
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports that neurons in the central nervous system can be induced to divide, and there are no reports on the use of synergistic effects of cytokines to form a prescription to treat nerve injury

Method used

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  • Formula constituted by cytokine and compound, its action of promoting nerve regeneration and action of researching and diagnosing nervous system disease
  • Formula constituted by cytokine and compound, its action of promoting nerve regeneration and action of researching and diagnosing nervous system disease
  • Formula constituted by cytokine and compound, its action of promoting nerve regeneration and action of researching and diagnosing nervous system disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Preparation of the prescription composed of cytokines and compounds of the present invention: take 1 mg of hydrocortisone, dissolve it in 10 ml of absolute ethanol, and prepare it as a 0.01% mother liquor; weigh 1 mg of progesterone, dissolve it in 10 ml of absolute ethanol, Prepare 0.01% mother liquor; weigh 100mg of insulin, dissolve in 10ml double-distilled water, and prepare 1% mother liquor; weigh 100mg of bovine serum albumin, dissolve in 10ml double-distilled water, and prepare 1% mother liquor; weigh Transferrin 2g, dissolved in 10ml double-distilled water, was prepared as 20% mother liquor; putrescine 300mg was dissolved in 10ml double-distilled water, and 3% mother liquor was prepared; 20mg triiodothyronine was weighed, dissolved In 10ml double-distilled water, prepare 0.2% mother liquor; weigh 1mg of sodium selenite, dissolve in 10ml double-distilled water, and prepare 0.01% mother liquor; dissolve 100μg EGF in 1ml double-distilled water, prepare 0.01% mother ...

Embodiment 2

[0089] Preparation of the prescription composed of cytokines and compounds of the present invention: dissolve 10 μg BDNF in 100 μl double distilled water to prepare a 0.01% mother solution; dissolve 10 μg NGF in 100 μl double distilled water to prepare a 0.01% mother solution; On the basis of the ingredients made in 1, BDNF was added to make the final concentration 2.5×10 -4 %; then add NGF to make the final concentration 5×10 -4 %. Mix evenly, make up to volume with double distilled water, and make the prescription of the present invention.

Embodiment 3

[0090] Example 3 Induced primary cortex and spinal cord neuron division experiment

[0091] Rat spinal cord and cortical neurons were isolated, and then a large dose of cytarabine was added to remove neural stem cells and other dividing cells. Then Nestin, GFAP and NSE immunocytochemical examination was used to confirm that there were no Nestin immunopositive cells, and primary neuron cells accounted for more than 95% of the total cells. Then add the recipe of cytokines and compounds obtained in Example 1 to induce neuron division. As a result (see FIG. 1 ), it was found that quite a lot of neurons were in the M phase (mitotic phase). The images show primary cortical and spinal cord neurons at different stages of mitosis.

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PUM

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Abstract

The invention discloses a formula of cell factor and compound and action to regenerate nerve and diagnose the nerve system, which is fit for insuline, bovine serum albumin, putrescine, hydrocortisone, luteohormone, transferrin, sodium selenite, alkaline cellulose cell growing factor and epiderm growing factor.

Description

Technical field: [0001] The present invention belongs to the field of nervous system diseases, and specifically uses the synergistic effect between cytokines and cytokines and compounds to construct a prescription of cytokines and compounds that can promote neuron division and have a strong nerve regeneration effect, and a composition of The application of prescriptions in the research, diagnosis and treatment of nervous system disorders. Background technique [0002] Nerve regeneration is difficult, the key is that neurons cannot divide. Whether neurons can divide is a big question in the life sciences. For a long time, neurons have been considered as terminally differentiated cells (Raedler, E. et al (1978). Anat. Embryol. 154, 267-312.; McConnell, S.K. (1988). BrainRes. Rev. 1,: 1- 23.; McKay, R.D.G. (1989). Cell 58, 815-821.; Pincus, D.W. et al. (1988). Neurosurgery, 42(4), 858-868). Generally, neurons break away from the cell cycle shortly after birth and lose the ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08A61K35/12A61P25/00A61P25/16A61P25/28A61K38/40C12N5/0793
Inventor 刘少君林秋霞丁爱石阙海平赵廷宝吕双红王国华杨树广
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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