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Pancreatic carcinoma cell lines with highly metastatic potential in the liver

A technique for pancreatic cell and liver metastasis, applied in the field of pancreatic cancer high liver metastasis cell line, can solve the problems of lack of experimental platform for pancreatic cancer liver metastasis, and achieve the effects of strong invasiveness, large metastasis range and high metastasis efficiency

Inactive Publication Date: 2007-08-01
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no model of liver metastasis of pancreatic cancer in China, and the research of liver metastasis of pancreatic cancer lacks a good experimental platform

Method used

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  • Pancreatic carcinoma cell lines with highly metastatic potential in the liver
  • Pancreatic carcinoma cell lines with highly metastatic potential in the liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Establishment of a human pancreatic cancer cell line with high liver metastatic potential

[0022] 1) Establishment of pancreatic cancer spleen xenograft tumor model: Digest SW1990 human pancreatic cancer cells in the logarithmic growth phase with trypsin, pipette into a single cell suspension, trypan blue staining shows that the ratio of viable cells is above 98%, adjust the concentration to 5 ×10 7 / ml. 0.4% pentobarbital sodium solution (45 mg / kg) intraperitoneally anesthetized nude mice, disinfected the abdomen, took a longitudinal incision at 0.5 cm to the left of the midline of the upper abdomen, and made a longitudinal incision with a length of about 8 mm. After entering the abdomen, find the spleen and expose the spleen And fix it, slowly inject 0.1ml of the above tumor cell suspension at the lower level of the spleen, pull out the needle and press to stop the bleeding, gently return the spleen to the abdominal cavity, and suture the abdominal wall mu...

Embodiment 2

[0026] Example 2 Application of SW1990HM Cell Line Spleen Transplantation Liver Metastasis Nude Mouse Model

[0027] 1) The conventional culture conditions in vitro are RPMI1640 culture medium containing 10% fetal bovine serum, 37°C, 5% CO 2 / 95% air, saturated humidity.

[0028] 2) SW1990HM cells grown to the logarithmic growth phase were routinely digested with 0.25% trypsin + 0.1% EDTA, the cells were washed with PBS, the living cells were counted by trypan blue exclusion method, and the cell suspension was prepared at a concentration of 5×10 7 / ml.

[0029] 3) Take 0.1 ml of the above cell suspension (5×10 6 ), inoculated into the spleens of 10 BALB / C-nu / nu male nude mice at the age of 4-6 weeks, and raised in a special pathogen-free environment.

[0030] 4) After 8 weeks of routine feeding, the nude mice were killed and soaked in 75% alcohol for 5 minutes for disinfection; aseptically operated, the nude mice were dissected, the liver was completely removed, and rinsed ...

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Abstract

The invention belongs to microbiological animal cell system fiels, which in detail relates to a pancreatic cancer hepatic metastases clone SW1990HM with hepatic metastases potence. The clone possesses special transition phenotype, and if inoculated to mouse spleen, it will result to 100% hepatic metastases, and it is the first human pancreatic cancer clone with hepatic metastases potence in domestic. The genetic backgroud of said clone strain is the same to that of origianl cell, and it is characterized by strong invasiveness, high transmission efficiency and large transmission range. The invention provides proper experimental platform for further researching, preventing and treating pancreatic cancer hepatic metastases.

Description

technical field [0001] The invention belongs to the field of microbial animal cell lines, in particular to a pancreatic cancer high liver metastasis cell line with high liver metastasis potential. Background technique [0002] The incidence of pancreatic cancer in my country has been on the rise in recent years, and most cases are diagnosed at an advanced stage. According to reports, 80% of patients have developed distant metastasis or local infiltration at the time of diagnosis, the survival period is 4-6 months, and the 5-year survival rate is less than 5%. Liver metastasis is very common in advanced pancreatic cancer. The incidence of liver metastasis is more than 50%, and the incidence of abdominal metastasis is 25%. The local recurrence rate of postoperative patients is also 50-80%. There is no effective treatment method yet. Liver metastasis of pancreatic cancer is a complex process, and the establishment of models of liver metastasis of pancreatic cancer is a key ste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N5/09
Inventor 刘鲁明石卫东
Owner FUDAN UNIV SHANGHAI CANCER CENT
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