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Method of preparing heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase

A technology of glucosidase and xylanase, applied in the direction of hydrolase, bacteria, etc., to achieve the effect of reducing initial hardness, specific hydrolysis substrate, and simple and easy method

Inactive Publication Date: 2009-12-30
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few relevant reports on thermophilic microorganisms and their enzyme production in China. Therefore, obtaining excellent thermophilic microorganism strains and conducting in-depth research on their enzyme production and properties have attracted more and more attention from researchers and producers.

Method used

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  • Method of preparing heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase
  • Method of preparing heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase
  • Method of preparing heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the preparation of thermostable xylanase

[0046] 1. Production of thermostable xylanase by solid fermentation

[0047] 1) Cultivation of Paecilomyes thermophila J18 for inoculation:

[0048] Paecilomyes thermophila (Paecilomyes thermophila) J18 (purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, with a preservation number of AS3.6885) seeds were streaked and inoculated from the preserved PDA slant on a newly prepared PDA slant or plate, and then Place the slant or plate in a 40-60°C incubator for static culture for 2-8 days. The spores on the inclined surface are washed with 20% glycerol solution by mass percentage, the washed spore liquid is used for solid fermentation to produce enzyme, and the grown plate is directly used for liquid fermentation.

[0049] 2) Production of xylanase liquid by solid fermentation

[0050] Paecilomyces thermophila J18 was used as fermentation strain and natural agr...

Embodiment 2

[0073] Embodiment 2, the preparation of thermostable β-xylosidase

[0074] 1. Production of thermostable β-xylosidase by solid fermentation

[0075] 1) Cultivation of Paecilomyes thermophila J18 for inoculation:

[0076] The seeds of Paecilomyces thermophila J18 (purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, with the preservation number AS3.6885) were streaked and inoculated from the preserved slant on the newly prepared PDA slant or plate, and then the slant or plate was placed Cultivate statically in a 40-60°C incubator for 2-8 days. The spores on the inclined surface are washed with 20% glycerol solution by mass percentage, the washed spore liquid is used for solid fermentation to produce enzyme, and the grown plate is directly used for liquid fermentation.

[0077] 2) Production of thermostable β-xylosidase by solid fermentation

[0078] Using Paecilomyces thermophilia J18 (purchased from the Culture Collecti...

Embodiment 3

[0111] Embodiment 3, the preparation of heat-resistant β-glucosidase liquid

[0112] 1. Production of thermostable β-glucosidase by solid fermentation

[0113] 1) Cultivation of Paecilomyes thermophila J18 for inoculation:

[0114] Paecilomyces thermophila J18 seeds were streaked and inoculated on a newly prepared PDA slant or plate from the preserved slant, and then the slant or plate was placed in a 40-60°C incubator for static culture for 2-8 days. The spores on the inclined surface are washed with 20% glycerol solution by mass percentage, the washed spore liquid is used for solid fermentation to produce enzyme, and the grown plate is directly used for liquid fermentation.

[0115] 2) Production of thermostable β-glucosidase by solid fermentation

[0116] The thermostable glucosidase was produced by solid fermentation with Paecilomyces thermophila J18 as the fermentation strain and natural agricultural waste wheat straw as the carbon source.

[0117] The medium of solid ...

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Abstract

The invention discloses a making method of heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase, which comprises the following steps: adopting agricultural waste as carbon source; fermenting Paecilomyces thermophila J18 at 40-60 deg.c to obtain the ferment liquid with heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase; purifying; obtaining electrophoretic grade product.

Description

technical field [0001] The invention relates to a method for preparing heat-resistant xylanase, heat-resistant β-xylosidase or heat-resistant β-glucosidase. Background technique [0002] Xylanase (Xylanase, EC 3.2.1.8) is an important class of hydrolase, which mainly acts on the β-1,4-glucosidic bond on the main chain of xylan in an endo-cutting manner, and the hydrolyzed products are mainly Oligosaccharides and xylose. At present, there are relatively many studies on xylanase of thermophilic fungi abroad. Haltrich et.al. (Enzyme Microb. Technol. 15: 854-860) used Schizophylum commune BT2115 to ferment microcrystalline cellulose as a carbon source. The carbohydrase activity reached 4839U / mL fermentation broth, which is the highest level reported so far for xylanase produced by liquid fermentation. There have been some research reports on fungal xylanase production in China, but there are few research reports on fungal xylanase production and thermophilic fungal strains. L...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/24C12N1/20
Inventor 江正强杨绍青闫巧娟王岚
Owner CHINA AGRI UNIV
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