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Method for rapidly detecting vitality of seeds

A detection method and seed technology, which is applied in the direction of chemical reaction analysis, chemiluminescence/bioluminescence, germination equipment, etc., can solve the problems of unclear luminescence mechanism, poor measurement accuracy, and limited application range, and achieve unlimited Vitality detection of damaged seeds, high accuracy, and the effect of less dosage

Inactive Publication Date: 2009-10-14
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the measurement time for identifying rice seeds is relatively long, and the time required is about 1 hour; and what is measured is the ultra-weak light emitted by rice seeds after absorbing water. The luminescence mechanism of this ultra-weak light itself is very unclear. Related to rice seed vigor remains to be studied
In addition, since the difference in seed water content will greatly affect this ultra-weak luminescence, this method has poor measurement accuracy and low sensitivity in principle, and it is easy to cause false images
At the same time, this method is only aimed at a single variety of rice, and its application range is extremely limited, so it cannot be extended to the detection of other seed vigor.

Method used

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  • Method for rapidly detecting vitality of seeds
  • Method for rapidly detecting vitality of seeds
  • Method for rapidly detecting vitality of seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0039] First, select rice seeds with different harvest years, the same size, and intact seed coats to be detected, soak these seeds in 1‰ mercury chloride solution for 2 minutes, and then wash them with distilled water for 3 times; put the cleaned whole seeds in a volume of 4 mL in a light-transmitting cuvette and placed in a dark box; use 2mL of CaCl with a concentration of 3mM 2Soak the seeds for 10 minutes; then add sea firefly fluorescein derivatives (MCLA) to a final concentration of 1 mM, then measure the chemiluminescence intensity with a single photon counter for 2 minutes; the measurement temperature is 30 (± 2) °C; figure 1 The average intensity of chemiluminescence of rice seeds in different middle harvest years mediated by MCLA, a fluorescein derivative of sea firefly. from figure 1 It can be seen that the chemiluminescence intensity of the rice seeds harvested in 2001, 2002, 2004, 2005 and 2006 is obviously different, and the chemiluminescence intensity decreases...

Embodiment 2

[0042] Firstly, select corn seeds with different harvest years, the same size, and intact seed coats to be detected, soak these seeds in 2‰ mercury chloride solution for 3 minutes, and then wash them with distilled water for 3 times; put the cleaned complete seeds in a volume of 5mL in a light-transmitting cuvette and placed in a dark box; use 3mL of CaCl with a concentration of 3mM 2 Soak the seeds for 10 minutes; then add sea firefly fluorescein derivatives (MCLA) to a final concentration of 1 mM, and measure the chemiluminescent intensity with a self-made single-photon counter for 2 minutes; the measurement temperature is 30 (± 2) °C; figure 2 The average intensity of chemiluminescence of maize seeds in different mid-harvest years mediated by the fluorescein derivative MCLA as the chemiluminescent reagent. from figure 2 It can be seen that the chemiluminescence intensity of corn seeds harvested in 2001, 2002, 2004, 2005 and 2006 is obviously different, and the chemilumin...

Embodiment 3

[0045] Firstly, soybean seeds harvested in August 2006 with the same size and intact seed coat were selected, and treated with high temperature (42° C.) and high humidity (100% relative humidity) for different times (the purpose was to reduce the vigor of the seeds). The seeds were soaked in 3‰ mercury liter solution for 1 min, and then washed three times with distilled water; the cleaned whole seeds were placed in a light-transmitting cuvette with a volume of 5 mL, and placed in a dark box; 3 mL of CaCl with a concentration of 3 mM 2 Soak the seeds for 5 minutes; then add sea firefly fluorescein derivatives (MCLA) to a final concentration of 1 mM, and measure the chemiluminescent intensity with a self-made single-photon counter for 2 minutes; the measurement temperature is 30 (± 2) °C; image 3 The average intensity of chemiluminescence of soybean seeds treated with medium-high temperature and high humidity for different periods of time mediated by MCLA, a fluorescein derivati...

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Abstract

The invention provides a kind of quick detection method of seed vigor, mainly under the same condition, measure the average value of the chemiluminescence intensity of the seed leachate in the early stage of imbibition of the seed to be tested under the mediation of a chemiluminescent probe reagent, and compare its size, thereby Realize the detection of the vigor size of the seeds to be tested. The invention requires a small amount of samples to detect seed vigor and has a wide application range; what is detected is the chemiluminescence of the seed leachate, which effectively eliminates the influence of other luminescent substances in the seeds and the water content of the seeds on the chemiluminescence, and can realize the seed vigor conveniently and quickly Non-destructive, accurate and highly sensitive detection.

Description

technical field [0001] The invention relates to a seed detection technology, in particular to a rapid detection method for seed vitality. Background technique [0002] Seed vigor is an important indicator of seed quality, and high vigor seeds have obvious growth advantages and production potential. In agricultural production, the determination of seed vigor is a necessary means to ensure the emergence rate and production potential in the field, and it is a key link to ensure high agricultural yield and harvest. [0003] Traditional seed viability detection is mainly to measure the germination rate (also known as germination potential), such as the hypertonic germination method, or to detect the stress resistance of seeds, such as the freezing germination method. The principle of the hypertonic germination method is that the germination ability of seeds in a hypertonic solution is significantly positively correlated with their vigor, and the vigor of the seeds is detected ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02G01N21/76
Inventor 邢达刘学俊张玲瑞
Owner SOUTH CHINA NORMAL UNIVERSITY
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