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Microbial fertilizer and its preparation process and application

A technology of microbial fertilizer and inoculation amount, applied in the direction of organic fertilizers and bacteria, can solve the problems of affecting the absorption of effective nutrients, destroying the balance of tobacco flora, and accelerating the death of tobacco, so as to inhibit the occurrence of insect pests, accelerate the rate of cell division, The effect of delaying the spread of germs

Active Publication Date: 2009-09-09
辽宁三色微谷科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no method that can cure the disease at all. Generally, it is sprayed with a sterilizing and disinfecting solvent. As a result, the balance of the flora in the tobacco growth environment is destroyed, not only does not have a therapeutic effect, but also affects the effective treatment of tobacco The absorption of nutrients, hastened the death of tobacco
Since the general fungicide raw materials are chemically synthesized components, it is easy to cause damage to the environment after spraying, and it is not easy to treat the environment after the damage

Method used

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  • Microbial fertilizer and its preparation process and application
  • Microbial fertilizer and its preparation process and application
  • Microbial fertilizer and its preparation process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The production conditions of each fermentation broth are shown in Table 1.

[0051] Among them, the light intensity of the photosynthetic bacterium Rhodopseudomonas palustris was 3000 lux during the cultivation process.

[0052] Table 1

[0053] Culture process

[0054] Rhodopseudomonas palustris Bacillus subtilis Streptomyces jingyang Lactobacillus acidophilus

[0055] 1 Inoculation amount (%) 20 15 10 5

[0056] Grade pH 6 7 8 6

[0057] Temperature (℃) 32 28 32 28

[0058] Cultivation time (days) 7 3 5 3

[0059] Inoculation amount (%) 35 5 20 20

[0060] Secondary pH 8 6 8 7

[0061] Cultivation temperature (°C) 28 30 32 28

[0062] Culture time (days) 7 2 5 2

[0063] Inoculation amount (%) 20 15 50 5

[0064] seed

[0065] pH 7 7 8 6

[0066] pot culture

[0067] Temperature (℃) 28 28 32 28

[0068] keep

[0069] Culture time (days) 7 3 5 3

[0070] ...

Embodiment 2

[0079] The production conditions of each fermentation broth are shown in Table 2.

[0080] Among them, the light intensity during the cultivation of the photosynthetic bacterium Rhodobacter sphaeroides was 4000 lux.

[0081] Table 2

[0082] bacteria

[0083] training process

[0084] Rhodobacter sphaeroides Bacillus cereus Streptomyces jingyang Lactobacillus plantarum

[0085] 1 Inoculation amount (%) 50 5 50 12

[0086] Grade pH 10 6 6 7

[0087] Temperature () 28 31 27 34

[0088] Cultivation time (days) 10 2 6 5

[0089] Inoculation amount (%) 20 15 50 5

[0090] Secondary pH 6 8 7 6

[0091] Cultivation temperature (°C) 36 28 27 34

[0092] Culture time (days) 10 5 6 5

[0093] Seed Inoculation amount (%) 50 5 20 20

[0094] pH 8 6 6 7

[0095] pot culture

[0096] Cultivation temperature (℃) 36 32 27 30

[0097] Culture time (days) 10 2 6 2

[0098] ...

Embodiment 3

[0106] The production conditions of each fermentation broth are shown in Table 3.

[0107] Among them, the light intensity of the photosynthetic bacterium Rhodopseudomonas palustris was 3500 lux during the cultivation process.

[0108] table 3

[0109] Culture process

[0110] Rhodopseudomonas palustris Bacillus glialis Streptomyces jingyang Lactobacillus acidophilus

[0111] 1 Inoculation amount (%) 35 8 30 20

[0112] Grade pH 8 8 7 8

[0113] Temperature (℃) 36 34 30 31

[0114] Cultivation time (days) 9 3 7 2

[0115] Secondary inoculum volume (%) 50 10 35 12

[0116] Culture pH 10 7 6 8

[0117] Temperature (℃) 32 32 32 31

[0118] Culture time (days) 9 3 5 3

[0119] Inoculation amount (%) 35 10 35 12

[0120] seed

[0121] pH 7 8 7 8

[0122] pot culture

[0123] Temperature (℃) 32 30 29 32

[0124] keep

[0125] Culture time (days) 8 3 7 3

[0126]...

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Abstract

The invention relates to a microbial fertilizer, which is prepared by mixing fermentation liquids of photosynthetic bacteria, bacillus, actinomycetes and lactic acid bacteria in proportion. The present invention also relates to the preparation method of the microbial fertilizer, which comprises inoculating the parent species of photosynthetic bacteria, bacillus, actinomycetes and lactic acid bacteria on the primary culture medium for cultivation to obtain the primary strain; respectively inoculating the above-mentioned primary strain Inoculate in the secondary culture medium for cultivation to obtain the secondary strains; inoculate the above-mentioned secondary strains in the fermentation medium for cultivation respectively to obtain the fermentation liquid; mix the fermentation liquid in proportion to obtain the obtained product. The present invention also relates to the application of the microbial fertilizer in the preparation and treatment of tobacco black neck disease, tree dryness, death, freezing damage and disease and its use method. The microbial fertilizer of the present invention can delay the spread of tobacco black neck disease bacteria, truly realize the biological control concept of using bacteria to control bacteria, and is a very economical and effective microbial fertilizer for increasing production.

Description

technical field [0001] The invention belongs to the field of fertilizers, and in particular relates to a microbial fertilizer and its preparation method and application. Background technique [0002] Tobacco black neck, caused by fungal infections of the flagellate subdivision Phytophthora. The fungus likes high temperature and humidity, and the optimum temperature for mycelial growth is 28-32°C. It occurs less at the seedling stage, and mainly harms the adult plants in the field, and both roots, stems and leaves can be affected. Some people call it "knocking the ground". There are the following manifestations during the onset, one of which is "wearing a coat": the stem expands to the pith after being injured, and wilts during the day. The second situation is "black neck": the fungus infects from the root or stem base, causing dark brown necrotic spots to appear on the root and stem base, and the aboveground part is chlorotic and dead. The third situation is "black plast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08C12N1/20
Inventor 王立平
Owner 辽宁三色微谷科技有限公司
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