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Chromium-rich saccharomyces cerevisiae, chromium-rich yeast product and their production process

A technology of chromium-enriched yeast and Saccharomyces cerevisiae, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low organic chromium content, low yield, low chromium utilization rate, etc., and reach organic chromium content High, stable chromium resistance, fast growth rate

Active Publication Date: 2009-02-25
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, there have been many studies on chromium-enriched yeast at home and abroad, but few can be mass-produced. The main problems are: (1) the chromium ion concentration and culture conditions of the medium for cultivating chromium-enriched yeast have not been optimized, or the optimization is not enough. The chromium content in the yeast is low, generally below 1000mg / kg, and the highest can only reach 4000mg / kg; (2) The organic chromium content is low, and some of them are simply adsorbed or mixed with yeast and chromium, and the inorganic chromium is not transformed It is organic chromium; (3) some methods are not suitable for large-scale production, the repeatability is very poor, and some can be produced, but the yield is very low; (4) the utilization rate of chromium is low

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the screening of saccharomyces cerevisiae strain of the present invention

[0045] 1) Inoculate yeast strains with different sources and uses into different concentrations of chromium-containing medium, and screen the strains that can grow in high-chromium medium. The strains resistant to chromium were selected as strains for mutagenesis.

[0046] 2) Inoculate the chromium-resistant strains on the culture medium containing different doses of lithium chloride for mutagenesis for 24 hours.

[0047] 3) Separating and screening the mutagenized yeast strains, using shake flasks and small fermenters for fermentation to select the ones with high chromium content, fast growth rate, high yield and high utilization rate of chromium on the medium containing chromium The high-chromium-resistant chromium-rich yeast strain is the high-chromium-resistant chromium-rich yeast strain C460.

[0048] 4) Screening medium formula

[0049] The wort medium has a sugar content ...

Embodiment 2

[0052] Example 2: Production of Chromium-enriched Yeast Products - Scheme 1

[0053] The production process for the production of chromium-rich yeast with high chromium content: slant culture → primary liquid seed cultivation → secondary liquid seed cultivation → fermenter seed cultivation → fermenter cultivation → separation → drying → packaging. The specific process is as follows:

[0054] 1) Slant strain culture: Inoculate high-chromium-resistant chromium-rich yeast strains into malt with a sugar content of 5-25% by weight, a chromium (chromium trichloride) concentration of 10 μg / ml, and a pH value of 4-6.5 Cultivate for 24-48 hours at 26-35°C on the slant of juice solids.

[0055] 2) First-level liquid seed culture: Inoculate 1 to 4 rings of yeast strains on the inclined plane to 100 to 500 ml of 5 to 25 wt % sugar content and 10 to 300 μg / ml of chromium (chromium trichloride) concentration , In a special culture bottle for wort with a pH value of 4-6.5, cultivate it at ...

Embodiment 3

[0062] Example 3: Production of Chromium-enriched Yeast Products - Scheme 2

[0063] The production process for the production of chromium-rich yeast with high chromium content: slant culture → primary liquid seed cultivation → secondary liquid seed cultivation → fermenter seed cultivation → fermenter cultivation → separation → drying → packaging. The specific process is as follows:

[0064] 1) Slant strain culture: Inoculate high chromium-resistant chromium-rich yeast strains into malt with a sugar content of 5-25% by weight, a chromium (chromium trichloride) concentration of 300 μg / ml, and a pH value of 4-6.5 Cultivate for 24-48 hours at 26-35°C on the slant of juice solids.

[0065] 2) First-level liquid seed culture: inoculate 1 to 4 rings of yeast strains on the slant to be equipped with 100 to 500 ml of sugar content of 5 to 25% by weight, chromium (chromium trichloride) concentration of 300 μg / ml, pH In a special culture bottle for wort with a value of 4-6.5, cultivat...

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Abstract

The present invention relates to one kind of chromium-rich Saccharomyces cerevisiae, chromium-rich Saccharomyces cerevisiae product and their production process. Of the chromium-rich Saccharomyces cerevisiae product, each kilogram contains chromium in 4000-8000 mg, and organochromium accounts for 90-99 wt% of the total chromium content. The process of producing chromium-rich Saccharomyces cerevisiae product includes the following steps: 1. fermenting the chromium-rich Saccharomyces cerevisiae of the present invention in culture medium containing chromium in 10-300 mcg / ml at 26-35 deg.c; and 2. drying the fermented Saccharomyces cerevisiae liquid to obtain the chromium-rich Saccharomyces cerevisiae product. The chromium-rich Saccharomyces cerevisiae product is safe and non-toxic, and may be used in medicine, food, feed and other fields.

Description

technical field [0001] The invention relates to a chromium-rich Saccharomyces cerevisiae, a chromium-rich yeast product obtained from the chromium-rich Saccharomyces cerevisiae and a production method thereof. Background technique [0002] Chromium is an indispensable trace element for human body and animals. It participates in the carbohydrate metabolism and fat metabolism of the body, and has many important physiological functions. Chromium deficiency can cause diseases such as diabetes and arteriosclerosis. Cataracts, hyperlipidemia, etc. are also related to long-term chromium deficiency. Although the human body's demand for chromium is very small, the phenomenon of chromium deficiency in modern human body is still common. It will promote the massive excretion of chromium remaining in the body, which will further aggravate the chromium deficiency. Epidemiological surveys show that the intake of chromium in the normal diet of modern people is often lower than the requir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/865
Inventor 俞学锋李知洪余明华姚娟张彦
Owner ANGEL NUTRITECH CO LTD
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