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Method for increasing yield of prodn. of hypericum japonicum total flavone

A technology of Tianjihuang and total flavonoids is applied in the pharmaceutical field, which can solve the problems of destroying the structure of flavonoids, decreasing the adsorption performance of macroporous resin, poor solubility, etc., and achieving the effect of improving solubility

Inactive Publication Date: 2008-12-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Flavonoids are weakly acidic because they often have multiple phenolic hydroxyl groups. Therefore, they have good solubility in alkaline aqueous solution. Using alkaline aqueous solution to dissolve and dilute the concentrated extract can solve the problem of poor solubility of flavonoids in water. However, if the solution is too alkaline, the adsorption performance of the macroporous resin will decrease, and at the same time, the structure of flavonoids may be destroyed, resulting in the loss of biological activity

Method used

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  • Method for increasing yield of prodn. of hypericum japonicum total flavone
  • Method for increasing yield of prodn. of hypericum japonicum total flavone

Examples

Experimental program
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Embodiment 1

[0013] Take 2 kg of Tianjihuang medicinal material coarse powder, add 6 times of water, heat and reflux for extraction once, each extraction time is 0.5 hours, combine the extracts, and centrifuge (rotating speed 2000, time 5 minutes); Concentrate the water extract to relative density 1.2, add 0.25% NH 3 ·H 2 O is dissolved and diluted to make the concentration to 0.25g crude drug / mL, pH to 4, centrifuge again, the supernatant is passed through a chromatographic column equipped with D101 type macroporous adsorption resin (non-polar), and the extract is prepared according to Tianjihuang The ratio of the amount of crude drug to the dry resin is 1:1, the column flow rate is 1BV / h, and the diameter-to-height ratio of the resin is 1:3; the resin is washed with water for 3BV, the elution flow rate is 1BV / h, and the water eluent is discarded; 2BV was eluted with 30% aqueous ethanol at a flow rate of 1BV / h, and the ethanol eluate was collected; the ethanol eluate was concentrated und...

Embodiment 2

[0015] Take 2 kg of decoction pieces of Tianjihuang medicinal material, add 12 times of 95% ethanol, heat and reflux extraction 3 times, each extraction time is 3.0 hours, combine the extracts, and centrifuge (rotating speed 10000 rpm, time 90 minutes); the ethanol extract Concentrate to a relative density of 1.35, add 1.0% NH 3 ·H 2 O is dissolved and diluted to make its concentration to 1.0g crude drug / mL, pH to 8, and centrifuged again. The supernatant is passed through a chromatographic column equipped with AB-8 type macroporous adsorption resin (weak polarity), and the extract is obtained by field The ratio of base yellow crude drug to dry resin is 1:5, the column flow rate is 4BV / h, and the diameter-to-height ratio of the resin is 1:9; the resin is washed with water for 12BV, and the elution flow rate is 6BV / h, and the water is discarded for elution. solution; elute with 6BV of 95% ethanol with water, the flow rate is 6BV / h, collect the ethanol eluate; concentrate the e...

Embodiment 3

[0017] Take 1 kg of decoction pieces of Tianjihuang medicinal material, add 8 times 50% ethanol, heat and reflux extraction twice, each extraction time is 2.0 hours, combine the extracts, filter; concentrate the ethanol extract to a relative density of 1.2, add 0.5% NH 3 ·H 2 O is dissolved and diluted to make the concentration to 0.5g crude drug / mL, pH to 6, centrifuge again, the supernatant is passed through a chromatographic column equipped with AB-8 type macroporous adsorption resin (weak polarity), and the extract is obtained by field The ratio of base yellow crude drug to dry resin is 1:3, the column flow rate is 2BV / h, and the diameter-to-height ratio of the resin is 1:5; the resin is washed with 6BV of water, the elution flow rate is 3BV / h, and the water is discarded. Eluate: elute with 4-6BV of 70% ethanol with water, the flow rate is 2BV / h, collect the ethanol eluate; concentrate the ethanol eluate under reduced pressure to a relative density of 1.1, and dry to obtai...

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Abstract

A process for increasing the output rate of the general flavone of Japanese St. John's-wort herb includes such steps as dissolving in NH3H2O solution, diluting the extracted concentrated liquid, and regulating the pH value of solution to become near neutral or slightly alkaline for increasing the solubility of flavone in water. Its output rate can reach 50-80%.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular discloses a 3 ·H 2 A method for improving the yield of total flavonoids produced by macroporous resin by applying O solution to the column. Background technique [0002] Tianjihuang is the dry whole herb of Hypericum japonicum Thunb in Garciniaceae. Sweet, slightly bitter, cool in nature. Return liver, gallbladder, large intestine channel. It has the effects of clearing heat and dampness, dissipating blood stasis and relieving pain, reducing swelling and detoxification. Modern pharmacological studies have shown that Tianjihuang has various pharmacological effects such as antibacterial, antiviral, liver protection, tumor and cancer cell inhibition, and immune enhancement. It is clinically used to treat acute and chronic hepatitis, infectious hepatitis, primary liver cancer, Typhoid fever, paratyphoid fever, etc. The total flavonoids are the material basis for the antibacterial, anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185C07H17/06C07D311/04
Inventor 田景奎唐丹张琳梁波李凌军
Owner ZHEJIANG UNIV
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