Method for obtaining hormone-free tissue culture detoxified seedling of chrysanthemum for tea use

Abstract

The invention discloses a method for producing nontoxic sprout of chrysanthemum for tea through non hormone tissue culture. It comprises the following steps: (1) getting the explant of stem top of chrysanthemum; (2) culturing the said explant and forming into clump sprout; (3) virus testing for clump sprout; (4) breeding the clump sprout; (5) root growing of the bred sprout and replanting. The method is characterized by the stable system, simple operation, high breeding rate and virus removing rate for tissue culture sprout, strong seedling, 50% lower cost than normal method, high quality of flower and simple management.

Description

technical field [0001] The invention belongs to a plant cultivation method, in particular to a method for obtaining virus-free seedlings through hormone-free tissue culture of tea chrysanthemums. Background technique [0002] The traditional propagation methods of tea chrysanthemum mainly use cuttings, divisions and other propagation methods, but on the one hand, these propagation methods require a large number of mother plants, and on the other hand, they also need to spend a lot of manpower, material resources, financial resources, time, etc., and the propagation speed is slow. For large-scale tea chrysanthemum production, the traditional propagation methods are far from meeting the needs of production. Simultaneously, due to the long-term use of asexual reproduction methods such as branching and cuttings, the plants are seriously infected with viruses (Cai Zhunan et al., 1992; Song Ruilin et al., 1997). At present, there are more than 10 kinds of viruses invading chrysan...

AI Technical Summary

Problems solved by technology

The above method does not apply to the edible requirements of chrysanthemum for tea