Method for reducing produced bacterial mud and mixture
A technology of bacteria slime and mixed liquid, applied in the field of methods and mixtures
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Embodiment 1
[0012] Example 1: Test of the influence of antagonistic bacteria and dispersants on the formation of deposits
[0013] Experimental procedure
[0014] Centrifuge 1mL of each strain isolated from the sludge cultured in NB liquid culture medium for 21-24 hours at 15000rpm for 1min, remove the supernatant, add 1mL of sterile water to resuspend all the test strains, and then inoculate into 100mL Erlenmeyer flask with sterilized white water and 3g LBKP slurry. Afterwards, different dispersants were added to the above treatment respectively, and cultured in a 40°C, 70rpm shaking incubator. After 3hrs of fully mixed culture, 3mL of the antagonistic bacteria C5 Streptomyces bikiniensis (Streptomyces bikiniensis) that had been cultured for 24h were added. Bacterial liquid, about 10 7 Bacterial count / ml, and cultivated in the same 40°C, 70rpm shaking incubator, observed and recorded the amount of precipitate produced after 7 days.
[0015] The whole experiment was divided into six t...
Embodiment 2
[0046] Embodiment two: Follow-up test of regular addition of antagonistic antibiotics
[0047] Experimental procedure
[0048]Centrifuge 1 mL of each strain isolated from the sludge cultured in NB liquid culture medium for 21-24 hours at 15,000 rpm for 1 min, remove the supernatant, add 1 mL of sterile water to resuspend all the test strains, and then inoculate into 100 mL of the sterilized Bacteria white water and 3g of LBKP slurry in the conical flask, fully mixed and cultured in 40 ° C, 70rpm shaking incubator, after 3 hours, add 3 mL of antagonistic bacteria C5 cultured for 24 hours, about 10 7 The number of bacteria / ml was also cultured in a 40°C, 70rpm shaking incubator, and the production of Deposit was observed and recorded every 7 days, and the antagonistic bacteria C5 was added at the same time, and the final results were observed and recorded after 14 days.
[0049] The whole experiment was divided into four treatments:
[0050] There are two comparison groups: ...
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