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Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist

A technology of antibacterial drugs and extracts, applied in the field of plant extracts, to achieve the effects of high product yield, enhanced bactericidal efficacy, and elimination of bacterial drug resistance

Inactive Publication Date: 2008-04-23
王秀英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the health care function of grapes has increasingly attracted the attention of scholars from all over the world in recent years, and extensive research has been carried out on its chemical composition, pharmacological effects, and development and application, but so far, no relevant grape extracts have been used as antibacterial drug synergists. research and application

Method used

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  • Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist
  • Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist
  • Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist

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Experimental program
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Effect test

Embodiment 1

[0051]A kind of grape extract, which is obtained by separating and extracting the fruit of grapes by water extraction, the roots, vines and leaves of grapes and grape skins by ethanol extraction, and the seeds of grapes by glycerin extraction. After purification by exchange method and chromatography, the obtained extracts are mixed evenly, and finally concentrated and dried, spray-dried and freeze-dried to obtain grape bistilbene, resveratrol, oleanolic acid and β-sitosterol , powdered extract of gallic acid and flavonoids.

Embodiment 2

[0052] Embodiment 2 separation and extraction

[0053] Soak raisin fruit in distilled water for 30 minutes, decoct in water at 100°C for 30 minutes, filter to remove residue, then separate and purify active substances by resin adsorption, and finally sterilize at 100°C for 10 minutes to obtain extract 1 After crushing grape roots, vines, and leaves, add ethanol, filter to remove slag, then separate and purify active substances through resin adsorption, and finally filter and sterilize to obtain extract 2; after soaking grape skins in distilled water, add acidic ethanol, filter Remove slag, then separate and purify the active substance through silica gel column chromatography, and finally filter and sterilize to obtain the extract 3; after crushing the grape seeds, add glycerin to extract at a temperature of 150°C, filter to remove slag, and then separate and purify by resin adsorption The active substance is finally filtered and sterilized to obtain the extract 4.

Embodiment 3

[0054] The mensuration of embodiment 3 active ingredients

[0055] The content of resveratrol was determined by HPLC; the content of flavonoids, β-sitosterol and gallic acid was determined by spectrophotometry, gas chromatography and thin-layer scanning.

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Abstract

The invention discloses a vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist, wherein the preparation consists of employing water extraction method, ethanol extraction method and glycerol extraction method to extract grape fruit, grape root, vine, leaf, peel and seeds, obtaining extract, mixing and concentration drying, spray-drying and freeze drying.

Description

Technical field: [0001] The present invention relates to a kind of plant extract, especially relate to the extract obtained from grape Vitis vinifera L.'s fruit, bark, root, vine, leaf, seed different parts; Meanwhile, the present invention also relates to the preparation method of this extract And its new application as synergist of antibacterial drugs. Background technique: [0002] Since Fleiming discovered penicillin in 1929 and Waksman discovered streptomycin in 1940, people have continuously found many effective antibiotics from microbial secondary metabolites, and thus developed many semi-synthetic antibiotics, which have played an important role in the prevention and treatment of bacterial infections. important role. Due to the long-term and extensive use of antibiotics, bacterial resistance has increased year by year, and once effective antibiotics can no longer inhibit or kill bacteria, which has become one of the main reasons for the decline in the efficacy of an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/87A61P31/04
Inventor 王秀英高荣黄鹤程朱冰张华平
Owner 王秀英
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