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Preparation method for transforming pineapple bran to biological feed stuff using microbe

A technology for microbial transformation and biological feed, which is applied in the field of microbial transformation of pineapple pomace into biological feed, can solve problems such as affecting the development of pineapple processing enterprises, and achieve the effects of eliminating bad pollution, making full use of resources and achieving golden color.

Inactive Publication Date: 2008-01-02
AGRI BIO TECH INST GUANGDONG ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Therefore, the treatment of pineapple pomace has seriously affected the further development of pineapple processing enterprises

Method used

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Examples

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Comparison scheme
Effect test

Embodiment Construction

[0022] The embodiment that a kind of microorganism fermentation pineapple pomace provided by the present invention produces biological feed is as follows:

[0023] The strains used in Example 1 are 2 strains of Pseudomonas stutzeri, 3 strains of Bacillus subtilis and 1 strain of Lactobacillus. The steps are:

[0024] A. the strain seed culture medium that is made up of 5 grams of glucose, 3 grams of lactose, 3 grams of sodium chloride, 5 grams of peptone, 5 grams of yeast extract and 1 liter of water (pH6.5) is poured into a sterile Erlenmeyer flask, prepared Seed liquid medium; LB liquid medium consisting of 3 grams of sodium chloride, 3 grams of peptone, 5 grams of yeast extract and 1 liter of water (pH6.5), poured into a sterile Erlenmeyer flask to make LB liquid medium The PYG liquid culture medium that is made up of 5 grams of glucose, 3 grams of lactose, 3 grams of sodium acetate, 5 grams of peptone, 5 grams of yeast extract and 1 liter of water (pH6.5) is poured into a...

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PUM

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Abstract

The invention discloses a process for preparing biological feeding-stuff by inverting pineapple bran with microorganism, inoculating a circular bacterial into seed culture medium, getting seed liquor by means of shake culture; inoculating Pseudomonas bacterial and bacillus subtilis into seed liquid in fluid enlarging LB medium with 1-5% inoculation consumption and inoculating lactic acid bacteria in PYG culture medium with 1-5% inoculation consumption, shake culturing, mixing every bacterial fermentation cultural liquid according to ratio of 1:1 as pineapple bran fermentation bacterial liquor; then inoculating which into pineapple bran, mixing evenly, compacting pineapple bran and loading into air-tight container for fermentation. The invention can eliminate environmental pollution caused by pineapple bran and at the same time produces green biological feeding-stuff of high nutritional value with the said waste.

Description

technical field [0001] The invention relates to a preparation method of using pineapple pomace as a raw material and utilizing microbial technology to transform and produce biological feed. Background technique [0002] Pineapple (Ananas comosus) is one of the tropical bulk fruits. It is nutritious, juicy, sweet and attractive, and is deeply loved by people; pineapple is widely planted in Guangdong Province, and a pineapple production of about 250,000 mu has been established in the Leizhou Peninsula. Base, pineapple is widely planted in southern China such as Fujian, Hainan, Guangxi and Southeast Asia. Pineapples have high water content and are difficult to store. Except for a small amount that are directly sold as fresh fruits, most pineapples are processed into canned pineapple, preserved fruit and other products and sold at home and abroad. [0003] With the continuous expansion of the pineapple planting area, various large and small pineapple processing enterprises have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23K1/14C12N1/20A23K10/12A23K10/30
Inventor 邝哲师张玲华徐志宏
Owner AGRI BIO TECH INST GUANGDONG ACADEMY OF AGRI SCI
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