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Process for preparing acrylamide aqueous solution through direct method cell enzyme reaction

A technology for acrylamide and cellular enzymes, which is applied in the field of direct-method cellular enzyme reaction to produce acrylamide aqueous solution, can solve the problems of long production cycle, complicated process, environmental pollution and the like, and achieves the effects of low cost, simple process and high product purity

Inactive Publication Date: 2007-08-29
江西昌九农科化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process is complicated, the production cycle is long, the cost is high, and there is certain pollution to the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 75ml of bio-fermentation liquid, separate it by centrifuge, the parameter is 7000rpm, control the chroma below 25, remove the supernatant, put the sediment into the reactor, then add deionized water to 725ml, adjust the pH value of the reaction system to 6 -8, the temperature was controlled at 15°C, and 300ml of chemically pure acrylonitrile was added to react for 240 minutes, and the reaction solution was quickly membrane-filtered, and the filtrate was taken to measure: AM% was 32.1% (mass ratio), AN% was 1.1 % (mass ratio), the conductivity is 1220 μs / cm, the chromaticity is 15, and the pH value is 6.8. Other methods for producing an aqueous solution of acrylamide by reacting with conventional cellular enzymes, for example, the conventional method for producing aqueous acrylamide solution provided by CN86100062A can be adopted.

Embodiment 2

[0034] Take 75ml of bio-fermentation liquid and separate it by membrane. The cut-off pore size parameter of membrane separation is 60,000 molecular weight, and the chromaticity is controlled to be below 15. Put the heavy phase into the reactor, add deionized water to 725ml, adjust the pH value to 6-8, and react The temperature of the system was controlled at 25°C, and 300ml of chemically pure acrylonitrile was added for reaction. After 240 minutes of reaction, the reaction solution was rapidly passed through membrane filtration, and the filtrate was taken to measure: AM% was 33% (mass ratio), AN% was 0.12 % (mass ratio); the conductivity is 500 μs / cm, the chromaticity is 10, and the pH value is 7.1. Other methods for producing an aqueous solution of acrylamide by reacting with conventional cellular enzymes, for example, the conventional method for producing aqueous acrylamide solution provided by CN86100062A can be adopted.

Embodiment 3

[0036] Take 75ml of bio-fermentation liquid and centrifuge in sequence at 8000rpm. The sediment is filtered through a membrane. The cut-off aperture parameter of the membrane separation is 50,000 molecular weight, and the chromaticity is controlled to be 5. The heavy phase is put into the reactor, and deionized water is added. to 725ml, adjust the pH value to 6-8, and control the ambient temperature at 18°C. Add 300ml of chemically pure acrylonitrile for hydration reaction. After 240 minutes of reaction, let the reaction solution pass through membrane filtration quickly, and take the filtrate to measure: AM % is 33% (mass ratio), AN% is not detected, the conductivity is 283 μs / cm, the chromaticity is 5, and the pH value is 7.5. Other methods for producing an aqueous solution of acrylamide by reacting with conventional cellular enzymes, for example, the conventional method for producing aqueous acrylamide solution provided by CN86100062A can be adopted.

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Abstract

The invention relates to a production method of acrylamide aqueous solution by direct cell enzyme reaction comprising: pretreating the fermentation liquor according to normal process to remove the impurities and make the chromaticity under 450, feeding the fermentation liquor into the reaction still cooling system, lowering the temperature, charging the acrylonitrile to react with the water. The invention is characterized in removing impurities in advance: that is, advancing the traditional subsequent impurities removal process of the acrylamide aqueous solution to the fermentation liquor pretreatment stage. So, the production method of acrylamide aqueous solution by cell enzyme reaction can not only be greatly simplified, but also save the cost, low the energy consumption, and improve the products quality.

Description

technical field [0001] The invention relates to a method for producing an aqueous solution of acrylamide by a cell enzyme reaction, in particular to a method for producing an aqueous solution of acrylamide by a direct cell enzyme reaction. Background technique [0002] Acrylamide is an important chemical raw material, which is usually produced by reaction in an aqueous solution medium, and its crystalline product is obtained through concentration, crystallization and drying processes. According to the patent literature (CN86100062A), the applicant: Nitto Chemical Industry Co., Ltd.'s invention "Method for Preparing Amides Using Microorganisms" suggests that the acrylamide aqueous solution generated by the free cell reaction of nitrilase and nitrile hydratase is used. The operation method is summarized as follows: select bacterial strains from microorganisms, cultivate fermentation broth, and then separate cells or solidify cells from the culture (fermentation broth). After ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/02C07C231/06C07C233/09
Inventor 顾保中杨中华黎常宏涂火中万真
Owner 江西昌九农科化工有限公司
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