The invention discloses a weeding composition containing cyhalofop-butyl, metamifop and pyriftalid, which comprises the following effective components: cyhalofop-butyl, metamifop and pyriftalid. The weight ratio of cyhalofop-butyl, metamifop and pyriftalid is (10-50):(10-50):(20-80). The weeding composition has a synergistic interaction or synergistic interaction function, can be used for preventing and killing off annual weeds, has effects remarkably superior to those of single dosage or dual mixed preparations, has an enlarged herbicide controlling spectrum, meanwhile reduces the pesticide usage amount, alleviates the pollution to the environment, delays the generation of weed resistance to pesticides, and is a very good novel medicine composition for preventing and killing off annual weeds in paddy fields.