Compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising peg moieties, useful for binding cells

Abstract

The present invention relates to novel compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG moieties, useful for binding cells, as well as uses and compositions related thereto. The compounds are useful for immobilizing and / or stabilizing cells.

Description

BRIEF SUMMARY OF THE DISCLOSURE[0001]The present invention relates to novel compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG moieties, useful for binding cells, as well as uses and compositions related thereto. The compounds are useful for immobilizing and / or stabilizing cells.BACKGROUND OF THE DISCLOSURE[0002]In US2005 / 0208644A1, a system employing two compounds is used for immobilizing cells. Disclosed therein is a method for immobilizing a cell in a desired pattern on a solid-phase surface by use of a chemical compound having an affinity for the cell. By using of another second chemical compound which is more easily immobilized on the solid-phase surface the first compound is bound to the second compound. The first chemical compound is described as a biocompatible anchor for membrane (BAM). This anchor has an aliphatic group which bines as it is inserted in the cell membrane and it can be immobilized by noncovalent bond without impairin...

AI Technical Summary

Benefits of technology

This patent is about new compounds that have a special structure made up of two parts: a hydrophobic part and a hydrophilic part. These compounds can be used to attach cells to surfaces, which helps to keep them stable and can also be used to make certain compositions. The technical effect of this invention is that it provides a new way to attach cells to surfaces and make related compositions.

Problems solved by technology

Cell immobilization using such compounds is neither quantitative nor cell-type independent.

Also, mixtures of different cell types, e.g. naturally occurring in a blood sample, cannot be attached to a surface quantitatively and independently of cellular phenotypes.

In addition binding of the cells to the surface is not tight enough for subsequent processing steps, e.g. immunochemical staining and washing.

Another disadvantage of the state-of-the art technology is that linker molecules described can either be internalized by cells or rejected by the cell, finally resulting in release and / or loss of cells.

It is disclosed in the paper that shear sensitivity of mammalian cells can be a problem impeding development of large scale animal cell cultivation.

However, cholesterol is a hydrophobic molecule and therefore it has to be dissolved in solvents like DMSO or alcohol which show cell toxicity at concentrations higher than 1% resulting in a limited cholesterol concentration which can be used to stabilize the cells.

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