Chip for optical analysis

a technology of optical analysis and chips, applied in the field of chips for optical analysis, can solve the problems of inability to perform pretreatment operations, inability to eliminate impurities, inability to perform simple measurement, etc., and achieve the effect of accurate liquid analysis, low cost and high sensitivity

Inactive Publication Date: 2014-12-11
NIPPON TELEGRAPH & TELEPHONE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about a chip that can easily analyze liquid samples by creating a reference sample from the liquid and detecting the substance to be measured in a real sample. The chip can prepare a reference sample with a free substance and selectively detect the measurement object in the real sample. It can also suppress signal noise caused by impurities and improve sensitivity and precision in measuring the substance. The chip can detect and quantify multiple substances with molecular selectivity and minimize changes in the baseline. Overall, the chip provides a convenient and accurate way to analyze liquid samples.

Problems solved by technology

However, when this reference sample is prepared, a complex pretreatment operation may be required, and such necessity of a pretreatment operation is not desirable in on-site or simple measurement.
In consideration of on-site measurement, such elimination treatment of impurities is not desirable either.
Such measurement by a total reflection optical system in which a probe molecule is immobilized on a substrate beforehand as described above suffers from a problem that many impurities are adsorbed or accumulated in a space on a substrate in which a measurement region is provided.
Therefore, a problem arises that, even if a difference between the reference region and the measurement region is deducted, effects of impurities cannot be eliminated, a baseline for measurement changes, and the baseline changes more greatly than a signal to be detected.
In a simple device without moving parts, however, it is impossible to detect a resonance angle in a condition where a sample is not introduced, and a signal cannot be obtained.
On the contrary, a problem arises that, even if a device has a mobile detection system, rapid measurement is impossible because the detection operation requires time.

Method used

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  • Chip for optical analysis
  • Chip for optical analysis
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Examples

Experimental program
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Effect test

example 1

[0154]This example shows a chip for optical analysis for a passage without a bypass passage as shown in FIGS. 2A and 2B, for which a protein A adsorbent is used as an adsorbent.

[0155]Cellulose acetate was dissolved in an organic solvent to prepare a cellulose acetate solution. A gold thin film (thickness 50 nm) was formed on a BK7 glass substrate by vapor-deposition. A resist membrane or a tape was applied onto this gold thin film, and a pattern in which a portion of the gold thin film corresponding to a passage was exposed was formed. The patterned gold / glass substrate was mounted on a spin coater, the cellulose acetate solution prepared beforehand was added dropwise to the substrate, then the substrate was rotated, and the organic solvent was evaporated to form a white membrane-form passage. Subsequently, a buffer solution of protein A was prepared and added dropwise to an adsorption region of the passage, and protein A was adsorbed to the passage to form the adsorption region. Th...

example 2

[0161]This example shows a chip for optical analysis for a passage without a bypass passage as shown in FIGS. 2A and 2B, for which a modified dextran microparticle adsorbent is used as an adsorbent.

[0162]A cellulose acetate solution obtained by dissolving cellulose acetate in an organic solvent and modified dextran microparticles (obtained from the microparticlation of methacrylate-modified dextran by radical polymerization) (diameter 15 to 30 μm) were mixed to prepare a cellulose acetate-dextran microparticle mixture solution. A gold thin film (thickness 50 nm) was formed on a BK7 glass substrate by vapor-deposition. A resist membrane or a tape was applied onto this gold thin film to form a pattern in which a portion of the gold thin film corresponding to a passage was exposed. At this time, an adsorption region of the passage on the metal thin film was prevented from being exposed, and the cellulose acetate solution was prevented from being deposited on the adsorption region. Subs...

example 3

[0168]This example is a chip for optical analysis with a passage having a bypass passage as shown in FIGS. 3A and 3B using a protein A adsorbent as an adsorbent.

[0169]Cellulose acetate was dissolved in an organic solvent to prepare a cellulose acetate solution. A gold thin film (thickness 50 nm) was formed on a BK7 glass substrate by vapor-deposition. A resist membrane or a tape was applied onto this gold thin film, and a pattern in which portions of the gold thin film corresponding to the main passage and the bypass passage were exposed was formed. The patterned gold / glass substrate was mounted on a spin coater, the previously prepared cellulose acetate solution was added dropwise, then the substrate was rotated, and the organic solvent was evaporated to form white membrane-form main passage and bypass passage. Subsequently, a buffer solution of protein A was prepared and added dropwise onto an adsorption region of the passage, and protein A was adsorbed to the passage to form the ...

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PUM

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Abstract

The present invention relates to a chip for optical analysis. In particular, the present invention relates to an optical sensor handling a liquid sample, which is a chip for analysis that can be used for selectively measuring a biologically-relevant substance or a chemical substance such as an environmental pollutant or a health affecting substance in a liquid to be measured. The chip for optical analysis of the present invention is characterized in that (1) an adsorption region (filter region) is provided between a sample introduction section and the observation section in a passage of the chip for analysis, (2) a bypass passage is provided in the passage (main passage) of the chip for analysis, and a time lag is generated between samples passed through the main passage and passed through a bypass passage, and (3) a measurement region and a reference region are provided in the observation section of the chip for analysis. In the present invention, the aspects (1) to (3) can be achieved individually or two or more thereof can be combined

Description

TECHNICAL FIELD[0001]The present invention relates to a chip for optical analysis. In particular, the present invention relates to a chip for analysis, which can be used for an optical sensor for handling a liquid sample that selectively measures chemical substances such as a biologically-relevant substance or an environmental pollutant or a health affecting substance in a liquid to be measured. The chip for analysis of the present invention can use a substance having molecular selectivity. Furthermore, the chip for analysis of the present invention measures a real sample containing impurities, with higher measurement sensitivity and higher precision in measurement. The chip for analysis of the present invention is intended for a simpler operation in measurement and higher sensitivity and higher precision in measurement.BACKGROUND ART[0002]The method for measuring a chemical substance in a liquid sample include for example electric and optical measurement methods in which a substanc...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/543G01N21/05G01N21/27G01N21/41
CPCG01N33/54373B01L3/502715B01L2200/148B01L2300/0645B01L2300/069B01L2300/0816B01L2300/0825B01L2300/0861B01L2300/0864B01L2400/084G01N21/553
Inventor SEYAMA, MICHIKOCAMOU, SERGEIWASAKI, YUZURUMIURA, TORUTAKAHASHI, JYUNICHIHAGA, TSUNEYUKIHORIUCHI, TSUTOMU
Owner NIPPON TELEGRAPH & TELEPHONE CORP
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