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Streptococcus vaccine compositions and methods of using the same

a technology of streptococcus and composition, applied in the direction of antibacterial agents, pharmaceutical active ingredients, antibacterial medical ingredients, etc., can solve the problems of ineffective current vaccine delivery system for a broad spectrum of diseases, inability to repeat immunization, and inability to effectively stimulate immune responses, so as to improve the translation of mrna, enhance the availability or accessibility, and different ligand binding profiles

Inactive Publication Date: 2014-07-10
RGT UNIV OF MICHIGAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides methods and compositions for stimulating immune responses against bacteria, particularly Streptococcus pneumoniae, using nanoemulsion compositions. The nanoemulsion compositions can contain various types of Streptococcus antigens, including but not limited to whole cells, lysates, and isolated proteins. The methods and compositions can be used for both therapeutic and preventative purposes. The invention is not limited to any particular strain or serotype of Streptococcus pneumoniae. The immunogenic compositions can be stable and can contain a pharmaceutically acceptable carrier. The invention provides a novel and effective way to induce immunity against bacteria and to protect against infections.

Problems solved by technology

Despite the availability of a variety of successful vaccines against many common illnesses, infectious diseases remain a leading cause of health problems and death.
Significant problems inherent in existing vaccines include the need for repeated immunizations, and the ineffectiveness of the current vaccine delivery systems for a broad spectrum of diseases.

Method used

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  • Streptococcus vaccine compositions and methods of using the same
  • Streptococcus vaccine compositions and methods of using the same
  • Streptococcus vaccine compositions and methods of using the same

Examples

Experimental program
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Effect test

example 1

Immunogenic Streptococcus pneumoniae Compositions

Experimental Design and Materials and Methods.

[0262]Outbred CD-1 or C57 / B6 (8 groups; 6 mice per group) were intranasally immunized with 7.5 μL or 0.14 WCPAg in 1, 5, 10 and 20% NE. WCPAg was generated as follows: strain RX1, a capsule-negative mutant derived from a pneumococcus capsular serotype 2 (e.g., an autolysin (lytA)-negative mutant of Rx1 (Rx1AL−)) grown at 37° C. in Todd-Hewitt broth supplemented with 0.5% yeast extract (THY) and 0.3 μg of erythromycin / ml to about 109 cells / ml, washed and suspended in saline at 10% of the original volume, and then mixed 3:7 (volume / volume) with ethanol, washed and resuspended in saline, and then frozen for later use.

[0263]The mice were given a volume of 12 μL (6 μL per nare) delivered manually into the nasal cavity of the mouse. Control groups were: 7.5 μL WCPAg in PBS, cholera toxin (CT), or Alum (delivered intramuscularly); 0.14 WCPAg in PBS; 20% NE alone.

[0264]The study design is presente...

example 2

Distribution of Anti-S. pneumoniae Antibody Titers at 8 Weeks after Two Vaccinations

[0278]FIG. 2 shows anti-S. pneumoniae IgG titer distributions at week 8, after two intranasal vaccine doses given one month apart on weeks 0 and 4, for experimental and control groups. All mice vaccinated with 108 CFU WCPAg plus varying concentrations of nanoemulsion obtained a serum antibody titer of 5×103 or greater, with a maximum of 105 and an average across all groups of 7×105. Titers for individual animals (circles) and mean serum titer per group (dash) are shown (See FIG. 2).

example 3

Distribution of Anti-S. pneumoniae Antibody Titers at 10 Weeks after Three Vaccinations

[0279]FIG. 3 shows Anti-S. pneumoniae IgG titer distributions at week 10, after three intranasal vaccine doses given one month apart on weeks 0, 4, and 8 for experimental and control groups. All mice vaccinated with 108 CFU WCPAg plus varying concentrations of nanoemulsion obtained a serum antibody titer of 5×103 or greater, with a maximum of 5×106 and an average across all 108 CFU / vaccine groups of 4×105. Titers for individual animals (circles) and mean serum titer per group (dash) are shown (See FIG. 3).

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Abstract

The present invention provides methods and compositions for the stimulation of immune responses. In particular, the present invention provides immunogenic nanoemulsion compositions and methods of using the same for the induction of immune responses (e.g., innate and / or adaptive immune responses (e.g., for generation of host immunity against a bacterial species of the genus Streptococcus (e.g., Streptococcus pneumoniae))). Compositions and methods of the present invention find use in, among other things, clinical (e.g. therapeutic and preventative medicine (e.g., vaccination)) and research applications.

Description

[0001]The present application is a divisional of U.S. patent application Ser. No. 13 / 320,205, filed on 13 Feb. 2012, which is a national phase application under 35 U.S.C. §371 of PCT International Application No. PCT / US2010 / 034999, filed on 14 May 2010, which claims priority to U.S. Provisional Patent Application Ser. No. 61 / 178,344 filed 14 May 2009, each of which is hereby incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention provides methods and compositions for the stimulation of immune responses. In particular, the present invention provides immunogenic nanoemulsion compositions and methods of using the same for the induction of immune responses (e.g., innate and / or adaptive immune responses (e.g., for generation of host immunity against a bacterial species of the genus Streptococcus (e.g., Streptococcus pneumoniae))). Compositions and methods of the present invention find use in, among other things, clinical (e.g. therapeutic and preventat...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/09
CPCA61K39/092A61K2039/521A61K2039/55566A61P31/04
Inventor BAKER, JR., JAMES R.MAKIDON, PAUL E.DUNLAP, WHITNEY A.KNOWLTON, JESSICA A.SWANSON, BENJAMIN
Owner RGT UNIV OF MICHIGAN
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