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Pharmacodiagnosis Test Targeting Oncology and Neurodegeneration

a neurodegeneration and drug therapy technology, applied in the field of medicine and biology, can solve the problems of less effective treatment and less broad implementation, and achieve the effect of better evaluating the treatment efficiency

Inactive Publication Date: 2013-12-05
FAURE LAURENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes a method for detecting cancer cells using certain proteins and genes. Specifically, the presence of proteins called protein kinase C epsilon (PKCs) and HDACs are indicative of cancer cells. The ratio of LIV21 to PKCs increases in cancer cells. The detection of certain genes like E2F1, E2F2, E2F3, and E2F4 can also indicate cancer cells. The use of these proteins and genes for diagnosis and treatment monitoring is advantageous, as they are involved in regulating gene expression and inhibiting cell proliferation. The invention is useful for evaluating the efficacy of treatments and making adjustments for individuals or groups with specific gene expression patterns.

Problems solved by technology

The consequence would be a less effective processing and of less broad implementation according to the heterogeneity of cancers.

Method used

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  • Pharmacodiagnosis Test Targeting Oncology and Neurodegeneration
  • Pharmacodiagnosis Test Targeting Oncology and Neurodegeneration
  • Pharmacodiagnosis Test Targeting Oncology and Neurodegeneration

Examples

Experimental program
Comparison scheme
Effect test

example 1

Extraction of Proteins of the Liv21 Complex.

The MCF-7 Cell Line

[0222]The MCF-7 line is a non clonal human line of breast denocarcinoma cells. During their differentiation induced by exogenous factors, these cells develop a hypertrophy, membrane protrusions and a tendency to dissociate from one another. They acquire a secretory phenotype which is characterized by the appearance of numerous granules and of secretory canaliculi.

[0223]In vivo, these cells are relatively non metastatic and this low invasiveness is thought to be due to a low constitutive activity of the protein kinases C (PKCs) and to a relatively low level of expression of protein kinase C alpha.

[0224]This line is used in many studies on proliferation, differentiation and apoptosis. These studies use appropriate drugs, such as TNF for the induction of apoptosis, or TPA (12-O-tetradecanoyl phorbol-13-10 sumoate) for the induction of differentiation and therefore for the study of departure from the cell cycle.

Extraction of...

example 2

Mass Spectrometry

[0226]A mass spectrometry (MALDI) was realized for the LIV21 protein and its complex. The LIV21 protein (polynucleotide Liv21F) was digested with trypsin. The peptides derived from the digestion are solubilized in a solvent: acetonitrile / water (1 / 1) containing 0.1% of TFA (trifluoroacetic acid). A saturated solution of the alpha-cyano-4-hydroxycinnamic matrix was prepared in the same solvent. The same volume of the two solutions was taken and mixed together and 1 μl was deposited onto the MALDI plate for analysis. The mass spectrometry showed that the LIV21 protein and its complex digested with trypsin reveals hundred peptides following the band of gel extracted between 49 and 54 kD (i.e. FIGS. 3-5). The LIV21 protein was characterized by a molecular weight of 50 kD revealed by Western blotting and by a two-dimensional SDS PAGE gel (FIG. 2). But we find a product of 100 kD at 130 kD which could be a Liv21 dimer. (I.e description FIGS. 4, 5 and 6)

[0227]When it change...

example 3

Analysis of Sequences in the Proteomic Databases

[0228]Several peptides in the patent in listing of the patent characterize it:

SEQ ID No 51Peptide LIV21b FVFALILALMLSMCGSEQ ID No 5Peptide LIV21b KFFVFALILALMLSMCGADSHAKR

[0229]For example, the inventor obtained a very significant score of 81, hoped: 0.0012 for the histatin 3-2 variant with 52% of overlapping of sequences between the tested sample and histatin, this sequence SEQ ID No5 is commune to Liv21 and HIS3-HUMAN (FIGS. 6 and 7). Using different database and a ppm which differed to 20 to 50 ppm, we obtain the same sequence for the commune part: SEQ ID No 51.

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Abstract

The present invention relates to the field of medicine and biology. It concerns a new test for screening and therapeutic follow-up in oncology. More particularly, it relates to diagnostic and / or therapeutic tests in oncology and on neurodegenerative diseases. Molecular targeting by peptide vectors and antibodies or by small interfering RNAs (siRNAs) opens a new concept of interdependence for diagnostic and therapeutic tools.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application is a continuation in part application of U.S. patent application Ser. No. 12 / 282,117 filed on Sep. 8, 2008. This application is also a continuation in part application of International Patent Application Serial No. PCT / FR2011 / 000155 filed on Mar. 18, 2011.FIELD OF THE INVENTION[0002]The present invention relates to the field of medicine and biology. It concerns a new test for screening and therapeutic follow-up in oncology. More particularly, it relates to diagnostic and / or therapeutic tests in oncology and on neurodegenerative diseases. Molecular targeting by peptide vectors and antibodies or by small interfering RNAs (siRNAs) opens a new concept of interdependence for diagnostic and therapeutic tools.[0003]The inventor highlights the mechanisms of molecular interactions and the interest of biochips dedicated according to cancers with a multi-therapy added with therapeutic additives to slow down the formation of these les...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113C07K14/47
CPCC12Q1/6886C07K14/47C12Q1/6883C12N15/113A61K31/7088A61K31/7105C12N2310/14C12Q2600/158C12Q2600/178C12N15/11
Inventor FAURE, LAURENCE
Owner FAURE LAURENCE
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