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Method of Blood Pooling and Storage

a technology applied in the field of blood pooling and storage, can solve the problems of damage to wbcs and inability to replica

Active Publication Date: 2012-10-04
NEW YORK BLOOD CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pathogen inactivation may additionally damage WBCs to render them unable to replicate.

Method used

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  • Method of Blood Pooling and Storage
  • Method of Blood Pooling and Storage
  • Method of Blood Pooling and Storage

Examples

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Effect test

example 1

[0059]Approximately 5 to 100 units of whole blood of the same type and group and leukoreduced and pooled. The pooled leukoreduced blood (RBCs, plasma, and platelets) is treated with UV radiation and optionally a type I and II quencher and / or radiation sensitizer to remove any pathogen and inactivate residual WBCs. If a quencher or radiation sensitizer is present, the cells are washed prior to continuing. The material is then fractionated into plasma, RBCs and platelets. A storage solution of adenine, glucose, sodium phosphate, mannitol and guanosine is added to the RBC component following pathogen inactivation. A filtering step is optionally added to remove poorly-deformable RBCs from any RBC-containing component. The resultant RBC-containing composition is further divided into RBC units comprising a uniform number and volume of RBCs / mL. The units are stored at 1°-6° C.

[0060]A storage solution is added to the platelet component following pathogen inactivation and the resultant plate...

example 2

[0062]The cell containing composition from Example 1 is analyzed for stability and viability of RBCs at a time period of 20 days, 40 days, 60 days and 100 days. Analysis of ATP and 2,3-DPG levels and percentage hemolysis as well as post-transfusion survival studies are used to determine the stability and viability of the RBCs in the cell containing solution. Storage life of the cell containing composition is determined therefrom.

example 3

[0063]About 100 units of blood are same blood group and type are individually subjected to a process of leukoreduction with a leukoreduction filter and subsequently fractionated via centrifugation for about 20 min at 2000 rpm. The isolated RBCs from each unit are washed with a phosphate buffered saline are and then pooled. The pooled RBCs are further subjected to UV radiation for about 2-4 hours or to inactivate any pathogenic contaminants. A storage solution is added to the RBCs and the RBCs are gently agitated by mechanical means to maintain the RBCs uniformly dispersed in the storage solution. The RBCs are divided into units having a uniform number of RBCs / mL. The units are stored at 1°-6° C. for about 42 to about 100 days prior to use.

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Abstract

The disclosure provides methods of making a red blood cell, plasma, and platelet products having a uniform dose and volume. The method comprises pooling a plurality of blood units, leukoreducing the blood and inactivating any pathogen contained therein. Plasma, RBCs, and platelets are then divided into uniform dose and volume units which have an extended shelf life.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The present application claims the benefit under 35 U.S.C. §119(e) to U.S. Provisional Application 61 / 649,121 filed May 18, 2012 and is a continuation-in-part of U.S. application Ser. No. 13 / 306,759 and International Patent Application PCT / US2011 / 62460, both filed Nov. 29, 2011, both of which claim the benefit under 35 U.S.C. §119(e) to U.S. Provisional Application 61 / 417,771 filed Nov. 29, 2010, the entire contents of all of which are incorporated by reference herein.BACKGROUND[0002]A single donation of whole blood can supply red blood cell (RBCs), platelets, and plasma products, which can potentially benefit three different patients. However, blood processing by blood banks is not optimized for quality and reproducibility of blood components. Stored RBCs suffer certain disadvantages. The lifespan of stored RBCs is 42 days, which given the fluctuation of supply and demand for RBCs, can lead to dangerous shortages in times of unexpected n...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N1/02
CPCA61K35/14A61M2202/0429A61M2202/20A01N1/0226A61M1/0272A01N1/02C12N5/0641A01K5/0114A01K5/02A01K5/0291A01N1/021A61K35/16A61K35/18A61K35/19A61M1/342A61M1/3496C12N5/0644
Inventor SHAZ, BETHHILLYER, CHRISTOPHER D.
Owner NEW YORK BLOOD CENT
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